大多数缺血性中风的体内模型以大脑中动脉和一系列中风严重程度为目标,从温和到实质性,可以实现。这篇综述描述了改善缺血性中风和动物福利的体内建模的机会。它提供了许多建议,以最大程度地减少大脑中动脉闭塞的最常见啮齿动物模型的严重程度。同时维持或改善科学成果。这些建议涵盖了手术前的基本要求,选择最合适的麻醉和镇痛方案,以及术中和术后护理。目的是为研究人员和动物护理人员提供支持,以完善他们的程序和实践,并实施微小的增量变化,以改善所使用动物的福利,并回答正在调查的科学问题。所有建议都在摘要海报中概述(见补充信息)。
Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).