在微卫星标记开发过程中,研究人员必须从一组可能的引物对中进行选择,以进一步测试他们感兴趣的物种。在许多情况下,目标是最大化可检测的遗传变异水平。为了指导研究人员并确定哪些标记与更高水平的遗传变异相关,我们基于1997-2012年发表的6782个基因组微卫星标记进行了文献综述.我们检查了具有以下标记特征的杂合性(He或Ho)或等位基因数(A)之间的关系:重复类型,图案长度,主题区域,重复频率,和微卫星大小。还分析了分类学组之间的差异。A和He的不完美重复类型和完美重复类型之间存在显着差异。二核苷酸基序表现出明显较高的A,He,和Ho比大多数其他图案。重复频率和基序区域与A呈正相关,He,和Ho,但与微卫星大小的相关性很小。较高的分类群体在文献中不成比例地代表,并且几乎没有一致性。总之,研究人员应该仔细考虑标记的特征,这样他们就可以根据所需的应用进行定制。如果研究人员的目标是高遗传变异,具有大重复频率的二核苷酸基序长度可能是最好的。
During
microsatellite marker development, researchers must choose from a pool of possible primer pairs to further test in their species of interest. In many cases, the goal is maximizing detectable levels of genetic variation. To guide researchers and determine which markers are associated with higher levels of genetic variation, we conducted a literature
review based on 6782 genomic
microsatellite markers published from 1997-2012. We examined relationships between heterozygosity (H e or H o) or allele number (A) with the following marker characteristics: repeat type, motif length, motif region, repeat frequency, and
microsatellite size. Variation across taxonomic groups was also analyzed. There were significant differences between imperfect and perfect repeat types in A and H e. Dinucleotide motifs exhibited significantly higher A, H e, and H o than most other motifs. Repeat frequency and motif region were positively correlated with A, H e, and H o, but correlations with
microsatellite size were minimal. Higher taxonomic groups were disproportionately represented in the literature and showed little consistency. In conclusion, researchers should carefully consider marker characteristics so they can be tailored to the desired application. If researchers aim to target high genetic variation, dinucleotide motif lengths with large repeat frequencies may be best.