目的:在慢性淋巴细胞白血病(CLL)中,某些特殊miRs的表达缺失或失调会破坏恶性细胞的凋亡;因此miR的表达可以增强细胞增殖,疾病进展和降低患者生存率。
结果:30名CLL患者和20名健康人参与了研究。提取RNA以评估miR-125,miR-223,BCL-2和信号转导和转录激活因子3(STAT3)基因的表达;进行定量实时PCR(Q-RT-PCR)。与对照组相比,患者的MiR-125a和miR-223表达降低(P值:0.001)。BCL-2和STAT3是这两种miR的靶基因,显示表达增加,患者与对照组相比(P值:0.001和P值:0.64)。miR-125a和BCl-2表达与WBC计数之间存在显著的反向关系。重要的是,miR-223表达与患者吸烟相关(P值:0.007)。此外,基于与白细胞计数和血红蛋白(Hb)浓度的负相关,这些miR可能通过控制白细胞(WBC)的产生而具有调节作用.最后,miR-223可用作CLL患者的预后因子;miR-125a可用于评估基于与BCl-2的反向链接的治疗方法。
OBJECTIVE: In chronic lymphocytic leukemia (CLL), lack of expression or dysregulation of some special miRs disrupts apoptosis of malignant cells; thereby miR expression can enhance cell proliferation, disease progression and decrease patient survival.
RESULTS: 30 CLL patients and 20 healthy individuals participated in the
study. RNA was extracted to evaluate the expression of miR-125, miR-223, BCL-2 and signal transducer and transcription 3 activator (STAT3) genes; quantitative Real Time- PCR (Q-RT-PCR) was performed. MiR-125a and miR-223 expression decreased in the patients compared to the control group (P-Value:0.001). BCL-2 and STAT3 which are the target genes of these two miRs, showed increased expression, in the patients compared to the control subjects (P-Value: 0.001 and P-Value: 0.64 respectively). A significant reverse relationship was found between miR-125a and BCl-2 expression and WBC count. Significantly, miR-223 expression was associated with smoking in patients (P-Value: 0.007). Also, these miRs may have regulatory effects by controlling white blood cell (WBC) production based on the inverse correlation with WBC count and hemoglobin (Hb) concentration. Finally, miR-223 can be used as a prognostic factor in CLL patients; miR-125a may be useful for evaluating the therapeutic approaches based on the inverse link with BCl-2.