marker-assisted breeding

标记辅助育种
  • 文章类型: Meta-Analysis
    CichoriumintybusL.是其属中经济上最重要的物种,也是菊科中最重要的物种之一。在菊苣,已经制作了许多连锁图,已经开发了几组映射和未映射的标记,研究了与农艺性状相关的数十个基因。这个信息宝库,适当地编目和组织,对于开发在产量和质量方面具有宝贵农艺潜力的优质商业产品至关重要,包括减少苦味和增加菊粉产量,以及对病原体的抗性或耐受性和对环境压力的抵抗力。出于这个原因,我们根据1980-2023年在菊苣上发表的科学文献进行了系统评价.根据荟萃分析的结果,我们创建了两个一致图谱,能够支持标记辅助育种(MAB)和标记辅助选择(MAS)计划.通过利用最近发布的C.intybus基因组,我们构建了一个基于639个分子标记的共有图谱,收集了该物种所有可用的作图和未作图的SNP和SSR基因座.在下一节中,在总结和讨论了菊苣中研究的所有基因以及与生殖障碍等感兴趣的特征相关的基因之后,倍半萜内酯生物合成,菊粉代谢和应激反应,我们制作了包含64个基因座的第二张图谱,这些基因座可用于MAS目的.随着组学技术的出现,分子数据混沌(即分子数据的数量如此复杂和难以管理,以至于它们的使用变得具有挑战性)的情况已不再是一个可忽略的问题。在这次审查中,因此,我们试图通过标准化和组织迄今为止在菊苣中产生的分子数据来促进育种者的工作。
    Cichorium intybus L. is the most economically important species of its genus and among the most important of the Asteraceae family. In chicory, many linkage maps have been produced, several sets of mapped and unmapped markers have been developed, and dozens of genes linked to traits of agronomic interest have been investigated. This treasure trove of information, properly cataloged and organized, is of pivotal importance for the development of superior commercial products with valuable agronomic potential in terms of yield and quality, including reduced bitter taste and increased inulin production, as well as resistance or tolerance to pathogens and resilience to environmental stresses. For this reason, a systematic review was conducted based on the scientific literature published in chicory during 1980-2023. Based on the results obtained from the meta-analysis, we created two consensus maps capable of supporting marker-assisted breeding (MAB) and marker-assisted selection (MAS) programs. By taking advantage of the recently released genome of C. intybus, we built a 639 molecular marker-based consensus map collecting all the available mapped and unmapped SNP and SSR loci available for this species. In the following section, after summarizing and discussing all the genes investigated in chicory and related to traits of interest such as reproductive barriers, sesquiterpene lactone biosynthesis, inulin metabolism and stress response, we produced a second map encompassing 64 loci that could be useful for MAS purposes. With the advent of omics technologies, molecular data chaos (namely, the situation where the amount of molecular data is so complex and unmanageable that their use becomes challenging) is becoming far from a negligible issue. In this review, we have therefore tried to contribute by standardizing and organizing the molecular data produced thus far in chicory to facilitate the work of breeders.
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  • 文章类型: Journal Article
    黄瓜花叶病毒(CMV)是影响辣椒品质和产量的流行病毒,在严重的局部流行病期间导致产量损失超过80%。文化习俗和大量使用农用化学品是CMV最常见的控制措施。抗性来源为CMV抗性育种提供了实际参考和依据。已经研究了CMV抗性的遗传因素,并通过传统育种方法开发了具有改良抗性的高级育种系和品种。此外,已经鉴定了CMV抗性的QTL或基因,可用于标记辅助的抗性育种。本文综述了CMV对不同病毒株的研究现状和前景,宿主抗性,及其应用遗传学。随着新技术的出现,更有用的标记和精确的方法可以促进提高辣椒CMV抗性的进展。
    Cucumber mosaic virus (CMV) is a prevalent virus affecting the quality and yield of pepper, resulting in yield losses of greater than 80% during severe local epidemics. Cultural practices and the heavy use of agrochemicals are the most common control measures for CMV. Sources of resistance provide a practical reference and a basis for breeding for CMV resistance. Genetic factors underlying CMV resistance have been studied and advanced breeding lines and cultivars with improved resistance have been developed by traditional breeding methods. Additionally, QTLs or genes for CMV resistance have been identified and can be utilized for marker-assisted resistance breeding. This review focuses on status and prospect of CMV against different virus strains, host resistance, and its applied genetics. With the advent of novel technologies, more useful markers and precise approaches can facilitate the progress for improving CMV resistance in Capsicum.
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