背景:最近根据形态学描述了唾液腺的乳腺类似物分泌癌(MASC),免疫组织化学,和分子(ETV6-NTRK3易位)与乳腺分泌性癌的相似性。MASC最重要的鉴别诊断考虑因素是未特别说明的低级别腺癌(NOS)。囊腺癌,和腺泡细胞癌(AcCC)。这些肿瘤可能与MASC具有重叠形态,和额外的免疫组织化学研究需要加强诊断。哺乳动物球蛋白,在MASC中已经报道了GCDFP-15和p63染色。我们的研究旨在检查与其他常见的唾液腺肿瘤相比,这些抗体在MASC中的特异性。
方法:一系列62例涎腺肿瘤[10例,5个NOS腺癌和2个具有MASC特征且无ETV6重排的囊腺癌,一个低级别筛状囊腺癌(LGCCC),9个酸性CC,10个MEC,10个腺样囊性癌(AdeCC),5个多形性低度腺癌(PLGA),和10个多形性腺瘤(PAs)]用乳腺球蛋白进行免疫组织化学分析,GCDFP-15和p63抗体。
结果:在所有的MASCs中都观察到乳腺球蛋白阳性,囊腺癌,LGCCC,和PLGAs,在一些腺癌NOS,PAs,和MEC,很少在AciCC中,从不在AdeCC中。在除AdeCC以外的大多数肿瘤类型中观察到GCDFP-15的阳性。有趣的是,在大多数MASCs和PLGAs中观察到p63的细胞质阳性,而在NOS和PAs中很少见,在其他类型的肿瘤中从未出现过。
结论:我们的研究揭示了乳腺球蛋白和p63细胞质染色的有用性,以确定哪些肿瘤值得筛选ETV6重排。
BACKGROUND: Mammary analog secretory carcinoma (MASC) of the salivary gland has been recently described according to morphological, immunohistochemical, and molecular (ETV6-NTRK3 translocation) similarities with the mammary secretory carcinoma. The most important differential diagnostic considerations of MASC are low-grade adenocarcinoma not otherwise specified (NOS), cystadenocarcinoma, and acinic cell carcinoma (AciCC). These tumors may share an overlapping morphology with MASC, and additional immunohistochemical studies are required to reinforce the diagnosis.
Mammaglobin, GCDFP-15, and p63 staining have been reported in MASC. Our
study was designed to check the specificity of these antibodies in MASC compared to other frequent tumors of salivary glands.
METHODS: A series of 62 salivary gland tumors [10 MASCs, 5 adenocarcinomas NOS and 2 cystadenocarcinomas with MASC features and without ETV6 rearrangement, one low-grade cribriform cystadenocarcinoma (LGCCC), 9 AciCCs, 10 MECs, 10 adenoid cystic carcinomas (AdeCCs), 5 polymorphous low-grade adenocarcinomas (PLGAs), and 10 pleomorphic adenomas (PAs)] was analyzed by immunohistochemistry with
mammaglobin, GCDFP-15, and p63 antibodies.
RESULTS: Positivity for
mammaglobin was observed in all MASCs, cystadenocarcinomas, LGCCC, and PLGAs, in some adenocarcinomas NOS, PAs, and MECs, rarely in AciCCs and never in AdeCCs. Positivity for GCDFP-15 was observed in most of the tumor types except in AdeCCs. Interestingly, cytoplasmic positivity for p63 was observed in most of MASCs and PLGAs while rarely in adenocarcinomas NOS and PAs, and never in the other tumor types.
CONCLUSIONS: Our
study revealed the usefulness of
mammaglobin and p63 cytoplasmic staining to define which tumors are worth to be screened for ETV6 rearrangement.