本研究旨在探讨常见心血管疾病危险因素和非编码RNA与原发性高血压（EH）的关系。
本研究共纳入402名EH患者和402名性别和年龄频率匹配的健康对照。每个参与者都接受了问卷调查，体格检查和实验室检查。进行定量实时聚合酶链反应（qPCR）以评估外周血白细胞中6种非编码RNA（NR_027032，NR_034083，NR_104181，miR-126，miR-143和miR-145）的相对表达水平。使用多元logistic回归分析来估计高血压和非高血压患者患EH的风险。
分析显示，有焦虑的参与者，高体重指数,腹型肥胖和高血压家族史有较高的EH风险,而那些饮食和职业体力活动平淡的人患EH的风险较低。qPCR检测显示，与对照组相比，EH患者的NR_027032(P=0.015)和NR_034083(P=0.004)显著降低，而NR_104181(P=0.007)，miR-143(P=0.005)和miR-145(P=0.015)显著升高。控制心血管危险因素后,多因素分析显示，NR_034083表达水平较低，NR_104181和miR-143表达水平较高是EH的危险因素。
EH是环境和表观遗传因素的结果。引人注目的是，NR_034083，NR_104181和miR-143可能与EH发展的风险相关；因此，表观遗传标记可用于测量高血压水平，以帮助阐明EH的发病机理。
The present study sought to explore the relationship of common cardiovascular disease risk factors and noncoding RNAs with essential hypertension (EH).
A total of 402 EH patients and 402 gender- and age-frequency matched healthy controls were enrolled in this study. Each participant received a questionnaire survey, physical examination and laboratory tests. Quantitative real-time polymerase chain reaction (qPCR) was performed to assess relative expression levels of six noncoding RNAs (NR_027032, NR_034083, NR_104181, miR-126, miR-143 and miR-145) in peripheral blood leucocytes. Multiple logistic regression analysis was used to estimate the risk of having EH between hypertensive and non-hypertensive patients.
Analysis showed that participants with anxiety, high body mass index, abdominal obesity and family history of hypertension had higher risk for EH, whereas those with bland diet and occupational physical activities had lower risk for EH. qPCR assays showed that NR_027032 (P = 0.015) and NR_034083 (P = 0.004) were significantly reduced in EH patients compared with controls, whereas NR_104181 (P = 0.007), miR-143 (P = 0.005) and miR-145 (P = 0.015) were significantly elevated. After controlling the cardiovascular risk factors, multivariate analysis showed that lower expression levels of NR_034083 and higher expression levels of NR_104181 and miR-143 were risk factors for EH.
EH is a result of environmental and epigenetic factors. Strikingly, NR_034083, NR_104181 and miR-143 may be correlated with the risk for EH development; therefore, epigenetic markers could be used to measure hypertension levels to help elucidate the pathogenesis of EH.
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