细胞分裂在叶片形态发生中起着不可或缺的作用,通过细胞周期蛋白和细胞周期蛋白依赖性激酶(CDK)形成的复合物进行调节。在这项研究中,基因家族分析,外源性生长素刺激,RNA-seq和WGCNA分析均用于研究细胞周期相关因子参与生长素信号通路对叶片形态发生的分子机制。鉴定并系统分析了白杨中的63个cyclin成员和17个CDK成员。在进化过程中,WGD是导致细胞周期蛋白和CDK基因扩增的主要原因。首先,在用生长素短时间处理成熟的幼苗叶片后,与GRF和ARGOS等细胞分裂相关的基因均上调,重新开始细胞从G1期向S期的转变.其次,连续三天对不同发育阶段的叶片进行生长素刺激,叶面积变化,分析了转录组和激素。根据PCA,PCoA和WGCNA分析,绿松石模块与叶片发育和生长素均呈正相关。基于共表达分析和Y2H实验,PoalbCYCD1;4,PoalbCYCD3;3和PoalbCYCD3;5被认为与PoalbCDKA;1相互作用,这可能是促进G1-S相变的触发因素。ARF转录因子可能通过影响CYC-CDK复合物在叶片形态发生中发挥连接生长素信号通路和细胞分裂的关键作用。
Cell division plays an indispensable role in leaf morphogenesis, which is regulated via the complexes formed by cyclin and cyclin-dependent kinase (CDK). In this
study, gene family analysis, exogenous auxin stimulation, RNA-seq and WGCNA analysis were all used to investigate the molecular mechanisms by which cell-cycle-related factors participated in the auxin signaling pathway on leaf morphogenesis. Sixty-three cyclin members and seventeen CDK members in Populus alba were identified and systematically analyzed. During the evolution, WGD was the main reason that resulted in the expansion of cyclin and CDK genes. Firstly, after a short time treating with auxin to matured leaves of seedlings, genes related to cell division including GRF and ARGOS were both upregulated to restart the transition of cells from G1-to-S phase. Secondly, with three days of continuous auxin stimulation to leaves at different developmental stages, leaves area variation, transcriptomes and hormones were analyzed. By PCA, PCoA and WGCNA analyses, the turquoise module was both positively related to leaf development and auxin. Based on the co-expression analysis and Y2H experiment, PoalbCYCD1;4, PoalbCYCD3;3 and PoalbCYCD3;5 were supposed to interact with PoalbCDKA;1, which could be the trigger to promote the G1-to-S phase transition. The ARF transcription factor might play the key role of connecting the auxin signaling pathway and cell division in leaf morphogenesis by affecting CYC-CDK complexes.