BACKGROUND: A long-term follow-up study was used to analyze the relationship between KISS1/GPR54 protein expression and the clinical prognosis of osteosarcoma (OS) patients.
METHODS: Forty-four paraffin-embedded OS samples conserved during the period from 2005 to 2009 were studied. Information about gender, age, pathological type, surgical stage, and clinical outcome was collected from the medical records.
RESULTS: This study included 25 males (56.81%) and 19 females (43.19%) with a median age at diagnosis of 18 years (range, 12-74 years). The follow-up duration ranged from 97 to 162 (121.36±15.46) months. The overall survival and metastatic rates were 43.18% and 65.91% respectively. For KISS1, the rate of distal metastasis and mortality in positive expression patients were 85.00% (17/20) and 75.00% (15/20) respectively, and 50.00% (12/24) and 41.67% (10/24) in negative expression patients respectively. In terms of survival time, KISS1 positive and negative patients were 55.35±14.29 and 97.46±13.06 months, respectively. P<0.05 was considered statistically significant in all three of the above aspects. For GPR54, the rate of metastasis and mortality of positive patients were 66.67% (24/36) and 61.11% (22/36), respectively, and 62.50% (5/8) and 37.50% (3/8) in negative expression patients. The survival time of GPR54 positive and negative expression patients were 73.97±11.81 and 92.75±15.42 months, respectively; however, P>0.05 in these three aspects.
CONCLUSIONS: Expression of KISS1 protein correlated with higher distal metastasis and shorter survival time in OS patients after an average of 10.11 years of follow-up. GPR54 protein did not affect the prognosis when expressed alone.

Polycystic ovary syndrome (PCOS) is the commonest endocrine disorder affecting young women. Kisspeptins are a family of closely related peptides encoded by Kiss1 gene that controls the hypothalamic-pituitary-gonadal axis by binding to its receptor (GPR54) expressed in gonadotropin-releasing hormone (GnRH) neurons and releases GnRH. Since GnRH secretion is deregulated in PCOS, we hypothesized that dysregulated gonadotropin secretion in PCOS is reflected by kisspeptin levels.
We aimed to measure serum kisspeptin levels of subjects with well-characterized PCOS versus controls and explore any correlation between kisspeptin and PCOS-related reproductive and metabolic disturbances.
: Consecutive women with PCOS manifesting from adolescence (n = 55) and adult controls (n = 110) were recruited. Pre-treatment baseline clinical, anthropometry, and biochemical parameters were measured in all. Serum kisspeptin and testosterone levels were determined by enzyme-linked immunosorbent assay method.
: Serum kisspeptin and testosterone concentrations were significantly higher in women with PCOS (kisspeptin 4.873 nmol/L; testosterone 4.713 nmol/L) than controls (kisspeptin 4.127 nmol/L; testosterone 3.415 nmol/L; P < 0.05). Serum kisspeptin levels were positively associated with PCOS (odds ratio: 1.853; 95% confidence interval: 1.246-2.755; P = 0.002) in our studied population.
Serum kisspeptin levels are higher in Sri Lankan women with PCOS manifesting from adolescence compared with controls regardless of body mass index. We propose serum kisspeptin concentration as a useful marker to recognize PCOS that manifests from adolescence.