目的:据报道,β-内酰胺抗生素中的一些在输注溶液和人体血液样品中会迅速降解。然而,目前血液样本中可用的稳定性数据仅限于少数β-内酰胺抗生素,并对相应研究的方法论进行讨论。本研究的目的是评估10β-内酰胺抗生素在人血浆样品中的稳定性。
方法:阿莫西林的稳定性,头孢唑啉,头孢吡肟,头孢噻肟,头孢西丁,头孢他啶,头孢曲松,亚胺培南,美罗培南,哌拉西林在20°C的低浓度和高浓度下进行了评估,4°C,-20°C,和-80°C持续1、7、60和90天,分别。
结果:阿莫西林,头孢吡肟,美罗培南,哌拉西林是最不稳定的抗生素。允许所有评估的β-内酰胺在两种测试浓度下的稳定性的最大持续时间估计为3小时,23h,10天,在20°C下35天,4°C,-20°C,和-80°C,分别。
结论:我们建议在4°C的冰中运输抗生素血浆样品,如果这些样品来自外部医院,甚至在-20°C。理想情况下,如果可能,血浆样品应储存在-80°C;如果不是,样品的分析应在-20°C储存后的10天内尽快进行。
OBJECTIVE: Beta-lactam antibiotics are reported for some of them to be subject to a rapid degradation in infusion solutions and in human blood samples. However, the current data of stability available in blood samples are limited to a few number of beta-lactam antibiotics, and the methodology of the corresponding studies may be discussed. The objective of the present
study is to evaluate the stability of 10 beta-lactam antibiotics in human plasma samples.
METHODS: Stability of amoxicillin, cefazolin, cefepime, cefotaxime, cefoxitin, ceftazidime, ceftriaxone, imipenem, meropenem, and piperacillin was evaluated at low and high concentrations at 20°C, 4°C, -20°C, and -80°C for 1, 7, 60, and 90 days, respectively.
RESULTS: Amoxicillin, cefepime, meropenem, and piperacillin were the least stable antibiotics. The maximum durations allowing the stability for all the evaluated beta-lactams at both tested concentrations were estimated at 3 h, 23 h, 10 days, and 35 days at 20°C, 4°C, -20°C, and -80°C, respectively.
CONCLUSIONS: We recommend to transport antibiotic plasma samples in ice at 4°C and even at -20°C if these samples come from external hospitals. Ideally, plasma samples should be stored at -80°C if possible; if not, the analysis of the samples should be performed as soon as possible in the limit of 10 days after a storage at -20°C.