体外胃肠模拟只有有限的方法来分析居住在粘膜区室的微生物群落。了解和区分肠道微生物生态系统对于更全面和准确地表示肠道微生物组及其与宿主的相互作用至关重要。这里建议,在短期和静态设置(名为“M-batches”)中,对人类结肠居民的粘膜和管腔种群的分析。在改变了几个参数之后,例如发酵体积和粪便接种物(单个或水池),仅发现微生物组成和代谢产生的微小差异。然而,由来自五个供体的粪便创建并以较小的体积(300mL)培养的水池似乎提供了更稳定的管腔生态系统。对M-batches中市售咖啡和绿茶的研究表明,这些全球已知饮料具有一些积极作用。包括产生丁酸的细菌和乳酸杆菌数量的增加。我们希望这种新策略可以促进肠道生态系统和宿主-微生物关系研究的未来进展,并有助于阐明微生物组在健康和疾病中的作用。
Gastrointestinal simulations in vitro have only limited approaches to analyze the microbial communities inhabiting the mucosal compartment. Understanding and differentiating gut microbial ecosystems is crucial for a more comprehensive and accurate representation of the gut microbiome and its interactions with the host. Herein is suggested, in a short-term and static set-up (named \"M-batches\"), the analysis of mucosal and luminal populations of inhabitants of the human colon. After varying several parameters, such as the fermentation volume and the fecal inoculum (single or pool), only minor differences in microbial composition and metabolic production were identified. However, the pool created with feces from five donors and cultivated in a smaller volume (300 mL) seemed to provide a more stable luminal ecosystem. The study of commercially available coffee and green tea in the M-batches suggested some positive effects of these worldwide known beverages, including the increase in butyrate-producing bacteria and lactobacilli populations. We hope that this novel strategy can contribute to future advances in the study of intestinal ecosystems and host-microbe relationships and help elucidate roles of the microbiome in health and disease.