不动杆菌属包括具有生态学意义的物种和机会病原体,并且具有复杂的分类学。精确的物种识别是理解细菌的基础。在这项研究中,我们发现并鉴定了两种新的不动杆菌,即,天福不动杆菌。11月。和隆辰不动杆菌。11月。,基于两种菌株WCHAc060012T和WCHAc060115T的表型检查和基因组分析。这两个菌株≤89.69%(平均值,79.28%或79.72%)平均核苷酸同一性(ANI)和≤36.4%(平均值,20.89%或22.19%)在硅DNA-DNA杂交(isDDH)值与彼此和所有已知的不动杆菌物种进行比较。两种物种都可以通过表型特征的组合与迄今为止已知的所有不动杆菌物种区分开。我们发现鸡只不动杆菌B301T和鲍氏不动杆菌AC877T实际上是同一物种,具有98.59%的ANI和90.4%的isDDH值。然后,我们应用更新的分类法来管理GenBank中的3,956例不动杆菌基因组,发现6%的不动杆菌基因组(n=234)需要校正或更新。我们鉴定出56种新的暂定不动杆菌属,将不动杆菌属物种的数量扩展到144个,其中包括68个具有物种名称的物种和76个未命名的分类单元。我们还发现,所有不动杆菌属和分类群的类型或参考菌株之间的ANI和平均氨基酸同一性(AAI)值≥76.97%和≥66.5%,分别,高于定义属边界的拟议截止值。这项研究强调了不动杆菌作为单个属的复杂分类学以及精确物种鉴定的重要性。新确定的未命名分类单元需要进一步研究。不动杆菌属在自然界中分布广泛,具有重要的生态学意义和临床意义。在这项研究中,首先,我们通过报道两种新的不动杆菌物种,显着更新了不动杆菌的分类学,即,天福不动杆菌和荣成不动杆菌,并通过鉴定鲍氏不动杆菌是鸡白肠不动杆菌的同义词。第二,我们使用更新的分类法,对保存在GenBank(n=3,956)中的不动杆菌基因组序列进行了整理,方法是对6%(n=234)基因组的物种分配进行校正,并将94(2.4%)分配到56个先前未知的试探性物种(分类群).因此,策展后,我们进一步更新了不动杆菌属,包括144种,包括68个物种名称和76个未命名的分类单元。第三,我们解决了是否应将如此大量的物种划分为不同属的问题,并发现不动杆菌确实是单个属。我们的研究大大推进了不动杆菌的分类学,具有科学和健康意义的重要属。
The genus Acinetobacter comprises species with ecological significance and opportunistic pathogens and has a complicated taxonomy. Precise species identification is a foundation for understanding bacteria. In this
study, we found and characterized two novel Acinetobacter species, namely, Acinetobacter tianfuensis sp. nov. and Acinetobacter rongchengensis sp. nov., based on phenotype examinations and genome analyses of the two strains WCHAc060012T and WCHAc060115T. The two strains had ≤89.69% (mean, 79.28% or 79.72%) average nucleotide identity (ANI) and ≤36.4% (mean, 20.89% or 22.19%) in silico DNA-DNA hybridization (isDDH) values compared with each other and all known Acinetobacter species. Both species can be differentiated from all hitherto known Acinetobacter species by a combination of phenotypic characteristics. We found that Acinetobacter pullorum B301T and Acinetobacter portensis AC 877T are actually the same species with 98.59% ANI and 90.4% isDDH values. We then applied the updated taxonomy to curate 3,956 Acinetobacter genomes in GenBank and found that 6% of Acinetobacter genomes (n = 234) are required to be corrected or updated. We identified 56 novel tentative Acinetobacter species, extending the number of Acinetobacter species to 144, including 68 with species names and 76 unnamed taxa. We also found that ANI and the average amino acid identity (AAI) values among type or reference strains of all Acinetobacter species and taxa are ≥76.97% and ≥66.5%, respectively, which are higher than the proposed cutoffs to define the genus boundary. This
study highlights the complex taxonomy of Acinetobacter as a single genus and the paramount importance of precise species identification. The newly identified unnamed taxa warrant further studies. IMPORTANCE Acinetobacter species are widely distributed in nature and are of important ecological significance and clinical relevance. In this
study, first, we significantly update the taxonomy of Acinetobacter by reporting two novel Acinetobacter species, namely, Acinetobacter tianfuensis and Acinetobacter rongchengensis, and by identifying Acinetobacter portensis as a synonym of Acinetobacter pullorum. Second, we curated Acinetobacter genome sequences deposited in GenBank (n = 3,956) using the updated taxonomy by correcting species assignations for 6% (n = 234) genomes and by assigning 94 (2.4%) to 56 previously unknown tentative species (taxa). Therefore, after curation, we further update the genus Acinetobacter to comprise 144 species, including 68 with species names and 76 unnamed taxa. Third, we addressed the question of whether such a large number of species should be divided in different genera and found that Acinetobacter is indeed a single genus. Our
study significantly advanced the taxonomy of Acinetobacter, an important genus with science and health implications.