我们对第一次产牛(AFC)的年龄进行了全基因组作图,目的是注释调节Nellore牛生育力的候选基因。使用了来自762头母牛的表型数据和来自2,992头公牛和母牛的777kSNP基因型。基于单步GBLUP方法的单核苷酸多态性(SNP)效应被阻断到1兆糖酶(Mb)的相邻窗口中,以解释遗传变异。在2、8、9、14、16和17号染色体上鉴定出解释超过0.40%的AFC遗传变异的SNP窗口。从这些窗户,我们确定了123个编码蛋白质基因,用于构建基因网络。从关联研究和衍生的基因网络来看,推定的候选基因(例如,PAPPA,准备,FER1L6,TPR,NMNAT1,ACAD10,PCMTD1,CRH,OPKR1,NPBWR1和NCOA2)和转录因子(TF)(STAT1,STAT3,RELA,E2F1和EGR1)与女性生育力密切相关(例如,促黄体生成素分泌的负调节,卵泡发生和子宫容受性的建立)。有证据表明,AFC遗传是复杂的,并受基因组中多个基因座的控制。由于在14号染色体上发现了几个解释遗传变异比例较高的窗口,因此应进行进一步的研究,以调查单倍型之间的相互作用,以更好地了解Nellore牛中AFC背后的分子结构。
We performed a genome-wide mapping for the age at first calving (AFC) with the goal of annotating candidate genes that regulate fertility in Nellore cattle. Phenotypic data from 762 cows and 777k SNP genotypes from 2,992 bulls and cows were used. Single nucleotide polymorphism (SNP) effects based on the single-step GBLUP methodology were blocked into adjacent windows of 1 Megabase (Mb) to explain the genetic variance. SNP windows explaining more than 0.40% of the AFC genetic variance were identified on chromosomes 2, 8, 9, 14, 16 and 17. From these windows, we identified 123 coding protein genes that were used to build gene networks. From the association
study and derived gene networks, putative candidate genes (e.g., PAPPA, PREP, FER1L6, TPR, NMNAT1, ACAD10, PCMTD1, CRH, OPKR1, NPBWR1 and NCOA2) and transcription factors (TF) (STAT1, STAT3, RELA, E2F1 and EGR1) were strongly associated with female fertility (e.g., negative regulation of luteinizing hormone secretion, folliculogenesis and establishment of uterine receptivity). Evidence suggests that AFC inheritance is complex and controlled by multiple loci across the genome. As several windows explaining higher proportion of the genetic variance were identified on chromosome 14, further studies investigating the interaction across haplotypes to better understand the molecular architecture behind AFC in Nellore cattle should be undertaken.