近几十年来,蓝藻水华已成为世界范围内的重要环境问题。然而,微囊藻毒素-LR(MC-LR)对水生生物的毒性效应,比如青蛙,仍然知之甚少。在这项研究中,青蛙(Pelophylaxnigromaculatus)暴露于环境相关浓度的MC-LR(0、1和10μg/L)21天。随后,我们评估了MC-LR对青蛙肝脏组织形态学的影响,并进行了基于MS的非靶代谢组学分析,然后测定与脂质代谢有关的物质。结果表明,MC-LR显着诱导了青蛙肝胰腺的组织学改变。鉴定出超过200种差异表达的代谢物,主要富含脂质代谢。生化分析进一步证实,MC-LR暴露导致青蛙脂质代谢紊乱。这项研究为机械理解青蛙和潜在的其他水生生物的MC-LR毒性奠定了基础。
Cyanobacterial blooms have emerged as a significant environmental issue worldwide in recent decades. However, the toxic effects of microcystin-LR (MC-LR) on aquatic organisms, such as
frogs, have remained poorly understood. In this study,
frogs (Pelophylax nigromaculatus) were exposed to environmentally relevant concentrations of MC-LR (0, 1, and 10 μg/L) for 21 days. Subsequently, we assessed the impact of MC-LR on the histomorphology of the
frogs\' livers and conducted a global MS-based nontarget metabolomics analysis, followed by the determination of substances involved in lipid metabolism. Results showed that MC-LR significantly induced histological alterations in the
frogs\' hepatopancreas. Over 200 differentially expressed metabolites were identified, primarily enriched in lipid metabolism. Biochemical analysis further confirmed that MC-LR exposure led to a disorder in lipid metabolism in the frogs. This study laid the groundwork for a mechanistic understanding of MC-LR toxicity in
frogs and potentially other aquatic organisms.