双醛羧甲基(DCMC)因其优异的生物相容性被选作最佳交联剂,在我们以前的研究中,适当的可降解性能和适当的化学反应性。然而,类似于其他传统的交联剂,DCMC固定的生物血管支架的内皮化不足。为了克服DCMC固定动脉中存在的这种限制,通过将SCPP引入动脉来研究锶掺杂多磷酸钙(SCPP)掺杂对DCMC固定动脉性能的影响,特别是固定动脉的内皮化。改性和交联后,它们的化学结构,机械性能,稳定性,并检查了细胞相容性。EDS分析结果表明SCPP已成功导入DCMC固定动脉。与纯DCMC交联的相比,DCMC/SCPP改性对固定动脉的机械强度无显著影响,但略有提高固定样品在D-Hanks溶液中的稳定性的倾向。此外,MTT测定,SEM观察和活/死实验表明,DCMC/SCPP修饰可以有效刺激HUVECs的粘附和增殖,从而促进固定动脉的内皮化过程。在这项研究中,首先将微米级的SCPP颗粒应用于DCMC交联猪动脉的改性,结果表明,该方法可以改善固定动脉的内皮化。因此,DCMC/SCPP修饰的动脉具有优异的理化性能和生物相容性,是一种有前途的血管支架材料。
Dialdehyde carboxymethyl (DCMC) was select as an optimal crosslinking reagent for its excellent biocompatibility, appropriate degradable property and suitable chemical reactivity in our previous research. However, similar to other traditional crosslinking reagent, the
endothelialization of biological vascular scaffolds fixed by DCMC was not sufficient enough. To overcome this limitation presented in DCMC-fixed arteries, a novel strategy was developed by introducing SCPP into arteries to investigate effects of strontium doped calcium polyphosphate (SCPP)-doping on the properties of DCMC-fixed arteries, especially on the
endothelialization of fixed arteries. After modifying and crosslinking, their chemical structures, mechanical properties, stability, and cytocompatibility were examined. The result obtained from EDS analysis indicated SCPP was successfully introduced into DCMC-fixed arteries. Compared with purely DCMC-crosslinked ones, DCMC/SCPP modification has no significant effect on mechanical strength of fixed arteries, but a slight tend to improve the stability of fixed samples in D-Hanks solution. Moreover, MTT assay, SEM observation and live/dead assay implied that DCMC/SCPP modification could effectively stimulate HUVECs\' adhesion and proliferation, and thus promote
endothelialization process of fixed arteries. In this
study, micron-sized SCPP particles were firstly applied to modify DCMC-crosslinked porcine arteries, and the results demonstrated this method could improve the
endothelialization of fixed arteries. Therefore, DCMC/SCPP-modified arteries with excellent physicochemical properties and biocompatibility should be a promising materials for fabricating vascular scaffolds.