■先前的研究表明,子宫内膜血管与妊娠之间存在显着关联,而血管形成不良归因于反复植入失败(RIF)。增强血管生成以成功植入的一种可能方法是子宫内膜刮伤(ES)。
■目的是通过分析不明原因的RIF参与者的血管生成相关基因表达来研究子宫内膜对抓挠的反应。
■在这项随机对照试验研究中,采用平衡区组随机法将20例原因不明RIF不孕妇女分为2组(每组10例):干预组(A组)(卵泡期接受ES治疗)和对照组(B组)。在分泌期进行子宫内膜活检。使用人血管生成生长因子的聚合酶链反应阵列试剂盒进行基因表达谱分析。还评估了植入和临床妊娠率。
■在血管生成促进基因中,FGF1,FGF13,FGF2,TGFA,ANG,ANGPT1和VEGFA显著上调(p<0.05)。IL12A(血管生成抑制细胞因子)显著上调(p<0.01)。相比之下,15个具有血管生成相关功能的基因,包括CXCL11、CXCL13、CXCL3、CXCL5、CXCL6、EREG、FIGF,FST,IL10,LEP,PPBP,PROK1,RHOB,TNF,和TYMP,在ES后下调。在植入方面,干预组(A组)和对照组(B组)之间没有显着差异(43.75%vs.28.57%)或临床妊娠率(75%vs.57.1%)。
■ES诱导血管生成相关基因表达的显著改变,关键血管生成/抗血管生成因子显著上调/下调。这些发现增强了我们对ES引发的分子反应的理解,强调ES对植入至关重要的血管生成复杂过程的潜在影响。
UNASSIGNED: A significant association between endometrial vascularity and pregnancy has been shown in previous research, while poor vascularization was attributed to repeated implantation failure (RIF). One possible approach to enhance angiogenesis for successful implantation is endometrial scratching (ES).
UNASSIGNED: The purpose was to investigate endometrial responses to scratching by profiling angiogenesis-related gene expression in unexplained RIF participants.
UNASSIGNED: In this randomized controlled
trial study, 20 infertile women with unexplained RIF were assigned to 2 groups by the balanced block randomization method (n = 10/each group): the intervention group (group A) (who received ES in the follicular phase) and the control group (group B). Endometrial biopsy was performed in the secretory phase. Gene expression profiling was performed using a polymerase chain reaction-array kit for human-angiogenic growth factors. The implantation and clinical pregnancy rates were also assessed.
UNASSIGNED: Among the angiogenesis-promoting genes, FGF1, FGF13, FGF2, TGFA, ANG, ANGPT1, and VEGFA were significantly upregulated (p < 0.05). IL12A (an angiogenesis-inhibiting cytokine) was significantly upregulated (p < 0.01). In contrast, 15 genes with angiogenesis-related functions, including CXCL11, CXCL13, CXCL3, CXCL5, CXCL6, EREG, FIGF, FST, IL10, LEP, PPBP, PROK1, RHOB, TNF, and TYMP, were downregulated after ES. No significant differences were observed between the intervention (group A) and control (group B) groups in terms of implantation (43.75% vs. 28.57%) or clinical pregnancy rates (75% vs. 57.1%).
UNASSIGNED: ES induced significant alterations in the expression of angiogenesis-related genes, with notable up/downregulation of key angiogenic/antiangiogenic factors. These findings enhance our understanding of the molecular responses triggered by ES, underscoring the potential influence of ES on the complex processes of angiogenesis crucial for implantation.