WistarKyoto大鼠(WKY)已被提议作为抑郁症的动物模型。去甲肾上腺素能细胞核,蓝斑(LC)和5-羟色胺能核,背中缝(DRN)广泛涉及该疾病的伦理病理学。因此,本研究的目的是研究WKY大鼠体内LC和DRN神经元的电生理特性,使用单单位细胞外技术。Wistar(Wis)和SpragueDawley(SD)大鼠用作对照品系。在来自WKY大鼠的LC中,基础放电率高于Wis和SD品系中获得的基础放电率,与Wis菌株相比,突发性放电活性也更大,而SD则没有。LC神经元对α2-肾上腺素受体激动剂的抑制作用的敏感性,与Wis相比,WKY大鼠的可乐定和抗抑郁药瑞波西汀较低,但不是SD。关于DRN神经元,WKY大鼠的爆发活性低于WIS和SD大鼠,尽管在其他射击参数中没有观察到差异。有趣的是,而DRN神经元对5-HT1A受体激动剂的抑制作用的敏感性,8-OH-DPAT在WKY菌株中较低,与Wis组相比,抗抑郁药氟西汀在该大鼠品系中的抑制效力更大.总的来说,这些结果指出了Wis和WKY大鼠之间关于去甲肾上腺素能和5-羟色胺能系统的重要电生理差异,支持WKY大鼠作为抑郁症细胞基础研究的重要工具的实用性。
The Wistar Kyoto rat (WKY) has been proposed as an animal model of depression. The noradrenergic nucleus, locus coeruleus (LC) and the serotonergic nucleus, dorsal raphe (DRN) have been widely implicated in the ethiopathology of this disease. Thus, the goal of the present
study was to investigate in vivo the electrophysiological properties of LC and DRN neurons from WKY rats, using single-unit extracellular techniques. Wistar (Wis) and Sprague Dawley (SD) rats were used as control strains. In the LC from WKY rats the basal firing rate was higher than that obtained in the Wis and SD strain, and burst firing activity also was greater compared to that in Wis strain but not in SD. The sensitivity of LC neurons to the inhibitory effect of the α2-adrenoceptor agonist, clonidine and the antidepressant reboxetine was lower in WKY rats compared to Wis, but not SD. Regarding DRN neurons, in WKY rats burst activity was lower than that obtained in Wis and SD rats, although no differences were observed in other firing parameters. Interestingly, while the sensitivity of DRN neurons to the inhibitory effect of the 5-HT1A receptor agonist, 8-OH-DPAT was lower in the WKY strain, the antidepressant fluoxetine had a greater inhibitory potency in this rat strain compared to that recorded in the Wis group. Overall, these results point out important electrophysiological differences regarding noradrenergic and serotonergic systems between Wis and WKY rats, supporting the utility of the WKY rat as an important tool in the research of cellular basis of depression.