背景:许多研究表明,牙科单元水线(DUWL)通常被广泛的微生物(细菌,真菌,原生动物)和以前的研究中已经报道了它的各种患病率。因此,本综述研究旨在描述DUWLs细菌生物膜污染的患病率。
方法:这是一项系统综述和荟萃分析,其中不同国际数据库中的相关关键词,包括Medline(通过PubMed)和Scopus进行了搜索。对检索到的研究进行筛选,并从纳入的研究中提取所需的数据。三种标准方法,包括美国牙科协会(ADA),疾病控制和预防中心(CDC)和污染的>100CFU/ml(C-100)标准被用于评估DUWL的细菌生物膜污染。所有计算DUWL细菌生物膜污染患病率的研究,和英文全文研究纳入荟萃分析。没有相关数据或使用异常实验室方法的研究被排除在外。通过相关的检查表评估了方法学上的偏差风险,最后,数据通过固定或随机效应模型汇集.
结果:确定并筛选了7136项研究,并将26项相关研究纳入荟萃分析。最古老的研究发表于1976年,最新的研究发表于2020年。根据ADA,CDC和C-100标准,细菌污染的患病率估计为85.0%(95%置信区间(CI):66.0-94.0%),77.0%(95CI:66.0-85.0%)和69.0%(95CI:67.0-71.0%),分别。DUWL中嗜肺军团菌和铜绿假单胞菌的患病率估计为12.0%(95CI:10.0-14.0%)和8.0%(95CI:2.0-24.0%),分别。
结论:本综述研究的结果表明DUWL中细菌生物膜的患病率很高;因此,建议使用适当的消毒方案,以降低污染的发生率,并减少可能的交叉感染.
Numerous studies have shown that dental unit water lines (DUWLs) are often contaminated by a wide range of micro-organisms (bacteria, fungi, protozoa) and various prevalence have been reported for it in previous studies. Therefore, this
review study aims to describe the prevalence of bacterial biofilm contamination of DUWLs.
This is a systematic
review and meta-analysis in which the related keywords in different international databases, including Medline (via PubMed) and Scopus were searched. The retrieved studies were screened and the required data were extracted from the included studies. Three standard methods including American Dental Association (ADA), The Center for Disease Control and Prevention (CDC) and contaminated > 100 CFU/ml(C-100) standards were used to assess the bacterial biofilm contamination of DUWLs. All studies that calculated the prevalence of bacterial biofilm contamination of DUWLs, and English full-text studies were included in the meta-analysis. Studies that did not have relevant data or used unusual laboratory methods were excluded. Methodological risk of bias was assessed by a related checklist and finally, the data were pooled by fixed or random-effect models.
Seven hundred and thirty-six studies were identified and screened and 26 related studies were included in the meta-analysis. The oldest included study was published in 1976 and the most recent study was published in 2020. According to the ADA, CDC and C-100 standards, the prevalence of bacterial contamination was estimated to be 85.0% (95% confidence interval (CI): 66.0-94.0%), 77.0% (95%CI: 66.0-85.0%) and 69.0% (95%CI: 67.0-71.0%), respectively. The prevalence of Legionella Pneumophila and Pseudomonas Aeruginosa in DUWLs was estimated to be 12.0% (95%CI: 10.0-14.0%) and 8.0% (95%CI: 2.0-24.0%), respectively.
The results of this
review study suggested a high prevalence of bacterial biofilm in DUWLs; therefore, the use of appropriate disinfecting protocol is recommended to reduce the prevalence of contamination and reduce the probable cross-infection.