目标:尽管牙齿受影响的频率很高,并且随着年龄的增长,牙囊(DF)中的病变频率增加,DF年龄变化尚不清楚。我们比较了DF中总体甲基化和羟甲基化与年龄的关系。
方法:从59名个体中获得与下第三磨牙相关的DF。通过ELISA评估全局DNA甲基化(5mC含量)和羟甲基化(5hmC)。我们测试了5mC和5hmC含量之间的相关性,以及每个人与患者年龄的相关性。年龄的差异,5mC,和来自男性/女性的DF中的5hmC,和位置(左/右下颌骨)进行了测试。
结果:59人的平均年龄为19.56±3.92,范围为13至31岁,大多数是女性(n=39)。5hmC含量与19岁以下年龄呈负相关(Spearman相关系数=-0.552,p=0.0003,n=38)。5hmC和5mC含量之间没有关系。年龄中位数没有差异(p=0.25),男性/女性之间的5hmC(p=0.33)和5mC(p=0.86),在年龄的中位数中也没有(p=0.39),5hmC(p=0.99)和5mC(p=0.22)在拔牙的左/右侧之间。
结论:建立了5hmC与年龄之间的负相关关系,DF中5mC和5hmC含量之间没有相关性。DF中总体DNA羟甲基化随年龄的降低的生物学意义仍有待澄清。
OBJECTIVE: Despite the high frequency of impacted teeth and increased frequency of lesions in dental follicles (DF) with aging, DF age-changes remain unclear. We compared the global methylation and hydroxymethylation profiles in DF in relation to age.
METHODS: DF associated with impacted lower third molars were obtained from 59 individuals. Global DNA methylation (5mC content) and hydroxymethylation (5hmC) were evaluated by ELISA. We tested the correlation between 5mC and 5hmC content, and the correlation of each with patients\' age. The differences in age, 5mC, and 5hmC in DF from men/women, and location (left/right mandible) was tested.
RESULTS: The mean age of the 59 individuals was 19.56 ± 3.92, ranging from 13 to 31 years, and most were women (n = 39). 5hmC content and age up to 19 years were inversely correlated (Spearman\'s correlation coefficient=-0.552, p = 0.0003, n = 38). There was no relationship between 5hmC and 5mC content. There was no difference in the medians of age (p = 0.25), 5hmC (p = 0.33) and 5mC (p = 0.86) between men/women, nor in the medians of age (p = 0.39), 5hmC (p = 0.99) and 5mC (p = 0.22) between the left/right side of the tooth extraction.
CONCLUSIONS: An inverse correlation between 5hmC and age was established, with no correlation between 5mC and 5hmC content in DF. The biological meaning of such a decrease of global DNA hydroxymethylation with age in DF remains to be clarified.