未经证实:慢性淋巴细胞白血病(CLL)是狗中最常见的白血病类型。它的特征是骨髓中肿瘤淋巴细胞的增殖,形态正常(成熟),但没有功能。犬的CLL通常起源于细胞毒性T淋巴细胞(TCD8),尽管关于免疫表型的预后价值存在争议,这种细胞谱系可能与良好的预后有关。
未经授权:10岁,整个女性,混合品种的狗被带到兽医学院大学医院(UdelaR)进行咨询,因为常规的术前检查显示淋巴细胞白细胞增多,正常细胞性贫血,和由寡克隆丙种球蛋白病引起的高球蛋白血症。超声显示脾肿大。对血液进行的PCR对犬埃里希菌呈阴性。进行血液和骨髓流式细胞术以补充诊断并进行免疫表型,显示CD8+T细胞谱系的CLL。脊髓造影证实了CLL的临床怀疑。建立了基于烷化剂和糖皮质激素的化疗治疗。到目前为止,患者总生存期为13个月,对治疗反应良好.
UNASSIGNED:免疫表型测试的组合,脊髓造影,血液学和生化特征证实我们的患者存在T-CLL.流式细胞术,越来越多地用于兽医学,使我们能够确认我们的患者中起源于细胞毒性T淋巴细胞的CLL的诊断,通过对犬物种CD45,CD3,CD5和CD8具有特异性的一级抗体的阳性染色以及对CD4,CD21和CD34的染色不存在。
Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in dogs. It is characterized by the proliferation of neoplastic lymphocytes in the bone marrow, which are morphologically normal (mature), but non-functional. CLL in canines commonly originates in cytotoxic T lymphocytes (TCD8+), and although there is controversy regarding the prognostic value of the immunophenotype, this cell lineage may be associated with a good prognosis.
A 10-year-old, entire female, mixed-breed dog was brought to the University Hospital of the Veterinary Faculty (UdelaR) for consultation because a routine pre-surgical check-up revealed lymphocytic leukocytosis, normocytic anemia, and hyperglobulinemia due to an oligoclonal gammopathy. The ultrasound revealed splenomegaly. PCR performed on blood was negative for Ehrlichia canis. Blood and bone marrow flow cytometry was performed to complement the diagnosis and carry out the immunophenotype, which showed CLL of CD8+ T-cell lineage. The clinical suspicion of CLL was confirmed by a myelogram. Chemotherapy treatment based on alkylating agents and glucocorticoids was established. So far, the patient has an overall survival of 13 months with a good response to treatment.
The combination of the immunophenotyping test, the myelogram, and the hematological and biochemical profile confirmed the presence of T-CLL in our patient. Flow cytometry, increasingly used in veterinary medicine, allowed us to confirm the diagnosis of CLL originating in cytotoxic T lymphocytes in our patient, through the presence of positive staining of primary antibodies specific for the canine species CD45, CD3, CD5, and CD8 and the absence of staining for CD4, CD21, and CD34.