N掺杂碳量子点(CQD)纳米粒子作为一种新型的纳米载体,稳定性,和高量子产率,以克服光动力疗法(PDT)中吲哚菁绿(ICG)障碍,同时具有细胞成像特性。对含有黑素瘤癌细胞的C57BL/6小鼠进行细胞培养研究和体内评估。结果显示ICG加载后的CQD尺寸从24.55nm略微增强至42.67nm。活性氧(ROS)测试的检测表明,CQD提高了ICG的光稳定性和ROS产生能力。细胞培养研究表明,ICG@CQD可以将B16F10细胞系的黑色素瘤细胞的存活率从纯ICG药物的48%降低到ICG@CQD的28%。通过共聚焦显微镜捕获的图像批准了ICG@CQD的更多细胞摄取和纳米载体的更合格的细胞成像能力。对含有黑素瘤癌细胞的C57BL/6小鼠的体内评估显示,与游离ICG相比,ICG@CQD对肿瘤生长的明显抑制作用。体内荧光图像证实ICG@CQD在肿瘤区域中的积累明显多于游离ICG。总之,ICG@CQD被提议作为一种创新的纳米载体,具有巨大的PDT和诊断潜力。
UNASSIGNED: Indocyanine Green (ICG) as an agent for photodynamic therapy (PDT) of melanoma cancer has low quantum yield, short circulation half-life, poor photo-stability, and tendency to aggregation.
UNASSIGNED: N-doped carbon quantum dot (CQD) nanoparticle was applied to encapsulate ICG and overcome ICG obstacle in PDT with simultaneous cell imaging property.
UNASSIGNED: CQD was prepared using hydrothermal method. Cell culture
study and In vivo assessments on C57BL/6 mice containing melanoma cancer cells was performed.
UNASSIGNED: Results showed that CQD size slightly enhanced from 24.55 nm to 42.67 nm after ICG loading. Detection of reactive oxygen species (ROS) demonstrated that CQD improved ICG photo-stability and ROS generation capacity upon laser irradiation. Cell culture
study illustrated that ICG@CQD could decrease survival rate of melanoma cancer cells of B16F10 cell line from 48% for pure ICG to 28% for ICG@CQD. Confocal microscopy images approved more cellular uptake and more qualified cell imaging ability of ICG@CQD. In vivo assessments displayed obvious inhibitory effect of tumor growth for ICG@CQD in comparison to free ICG on the C57BL/6 mice. In vivo fluorescence images confirmed that ICG@CQD accumulates remarkably more than free ICG in tumor region. Finally, ICG@CQD was proposed as an innovative nanocarrier for PDT and diagnosis.