目的:本研究旨在探讨雷沙吉兰对盲肠结扎穿孔所致脓毒症大鼠急性肺损伤的影响。
方法:将大鼠分为以下6组,第1组:Sham,组2:假+雷沙吉兰4毫克/千克,第三组:脓毒症,第4组:脓毒症+雷沙吉兰1mg/kg,第5组:脓毒症+雷沙吉兰2mg/kg,第6组:脓毒症+雷沙吉兰4mg/kg。用16号针穿过结扎点远端的盲肠打开总共四个孔。
结果:雷沙吉兰治疗脓毒症后谷胱甘肽水平和超氧化物歧化酶活性升高,丙二醛水平降低。2mg/kg和4mg/kg的剂量有统计学上显著的改善。雷沙吉兰也增加了Tnf-α,IL1β,IL6、NF-κβ和HMGB1基因表达在2mg/kg和4mg/kg剂量下呈剂量依赖性。在增加剂量的情况下,雷沙吉兰预防水肿的发展,炎症的形成,和出血。
结论:雷沙吉兰对盲肠结扎穿孔大鼠急性肺损伤具有抗氧化和抗炎作用。
OBJECTIVE: We aimed to investigate the effects of rasagiline on acute lung injury that develops in the sepsis model induced with the cecal ligation and puncture in rats.
METHODS: The rats were separated into the following six groups, Group 1: Sham, Group 2: Sham + Rasagiline 4 mg/kg, Group 3: Sepsis, Group 4: Sepsis + Rasagiline 1 mg/kg, Group 5: Sepsis + Rasagiline 2 mg/kg, Group 6: Sepsis + Rasagiline 4 mg/kg. A total of four holes were opened with a 16-gauge needle through the cecum distal to the point of ligation.
RESULTS: Rasagiline treatment increased glutathione level and superoxide dismutase activity while decreased the malondialdehyde level after the sepsis. There was a statistically significant improvement in the doses of 2 mg/kg and 4 mg/kg. Rasagiline also increased Tnf-α, IL1β, IL6, NF-κβand HMGB1 gene expressions in dose-dependent at 2 mg/kg and 4 mg/kg doses. In increased doses, rasagiline prevent the development of edema, the formation of inflammation, and hemorrhage.
CONCLUSIONS: Rasagiline exerts both antioxidant and anti-inflammatory effects on the cecal ligation and puncture induced acute lung injury in rats.