Coal mine in arid and semi-arid area is one of the most severely degraded ecosystems on the earth. The continuous decrease in groundwater level caused by coal mining will inevitably affect biogeochemical environment of the vadose zone, and then lead to the replacement of surface vegetation. Yimin open-pit coal mine was taken as an example to reveal the relationship between the groundwater depth and soil water content (SWC), soil salt content, soil electrical conductivity (SEC), soil organic matter (SOM), soil available potassium (SAK), soil available nitrogen (SAN), vegetation coverage, aboveground biomass and species richness. The results show that, the change of groundwater depth can affect soil properties and then change the characteristics of surface vegetation, and the change of surface vegetation can also react on soil properties. Vegetation coverage and aboveground biomass are negatively correlated with groundwater depth, and positively correlated with SWC, SEC, SOM and SAK. The shallow groundwater table is conducive to the accumulation of SOM, so that the surface biomass and vegetation coverage are high. The higher the surface biomass, the more the SAN is absorbed. Under natural conditions, the relative strength of biological nitrogen fixation and plant absorption determine the content of SAN. In the research area, when the depth of groundwater is less than 0.4 m will cause soil salinization, then lead to low species richness; Species richness is exponentially correlated with groundwater depth and decreases with the increase in groundwater depth.

The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 microM ethidium bromide for improving DNA fragment separation. Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for > 50 analyses before degradation and loss of resolution. The results are comparable to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining.