本研究调查了大麻叶输注(CSI)调节与癌细胞存活有关的主要代谢的能力,以及诱导人乳腺癌(MCF-7)细胞死亡。MCF-7细胞系用CSI处理48小时,阿霉素作为标准的抗癌药物,而未处理的MCF-7细胞作为对照。CSI在最高剂量下引起21.2%的细胞生长抑制。对照细胞的液相色谱-质谱(LC-MS)分析显示存在碳水化合物,维生素,氧化,脂质,核苷酸,和氨基酸代谢产物。用CSI治疗导致这些代谢物消耗了91%,同时产生硒代蛋氨酸,l-胱氨酸,脱氧腺苷三磷酸,循环AMP,硒蛋氨酸,三磷酸肌苷,磷酸腺苷硫酸盐,5\'-甲硫腺苷,尿酸,丙二酸半醛,2-甲基鸟苷,神经节苷脂GD2和丙二酸。通过代谢产物途径富集的代谢组学分析揭示了与葡萄糖相关的关键代谢途径的激活,脂质,氨基酸,维生素,和核苷酸代谢。CSI导致葡萄糖完全失活,维生素,和核苷酸代谢,同时灭活与癌细胞存活相关的关键脂质和氨基酸代谢途径。流式细胞术分析揭示了用CSI处理的MCF-7细胞中的凋亡和坏死的诱导。CSI的高效液相色谱(HPLC)分析显示存在大麻二酚,芦丁,肉桂酸,和Ferulic.这些结果描绘了CSI作为治疗和管理乳腺癌的替代疗法的抗增殖潜力,如通过其对葡萄糖的调节所描绘的。脂质,氨基酸,维生素,和核苷酸代谢,同时在MCF-7细胞中诱导细胞死亡。
The present
study investigated the ability of Cannabis sativa leaves infusion (CSI) to modulate major metabolisms implicated in cancer cells survival, as well as to induce cell death in human breast cancer (MCF-7) cells. MCF-7 cell lines were treated with CSI for 48 h, doxorubicin served as the standard anticancer drug, while untreated MCF-7 cells served as the control. CSI caused 21.2% inhibition of cell growth at the highest dose. Liquid chromatography-mass spectroscopy (LC-MS) profiling of the control cells revealed the presence of carbohydrate, vitamins, oxidative, lipids, nucleotides, and amino acids metabolites. Treatment with CSI caused a 91% depletion of these metabolites, while concomitantly generating selenomethionine, l-cystine, deoxyadenosine triphosphate, cyclic AMP, selenocystathionine, inosine triphosphate, adenosine phosphosulfate, 5\'-methylthioadenosine, uric acid, malonic semialdehyde, 2-methylguanosine, ganglioside GD2 and malonic acid. Metabolomics analysis via pathway enrichment of the metabolites revealed the activation of key metabolic pathways relevant to glucose, lipid, amino acid, vitamin, and nucleotide metabolisms. CSI caused a total inactivation of glucose, vitamin, and nucleotide metabolisms, while inactivating key lipid and amino acid metabolic pathways linked to cancer cell survival. Flow cytometry analysis revealed an induction of apoptosis and necrosis in MCF-7 cells treated with CSI. High-performance liquid chromatography (HPLC) analysis of CSI revealed the presence of cannabidiol, rutin, cinnamic acid, and ferulic. These results portray the antiproliferative potentials of CSI as an alternative therapy for the treatment and management of breast cancer as depicted by its modulation of glucose, lipid, amino acid, vitamin, and nucleotide metabolisms, while concomitantly inducing cell death in MCF-7 cells.