癌细胞外渗是癌症转移的关键步骤。然而,这个过程中涉及的许多机制现在才被阐明。因此,在本研究中,我们通过应用于transwell小室侵袭试验的高通量无标记细胞阻抗试验分析了黑色素瘤细胞的跨内皮侵袭.该技术实时监测和量化恶性肿瘤细胞对内皮细胞的侵袭,很长一段时间,避免由于准备终点测量而造成的伪像。将通过阻抗分析获得的结果与终点测量值进行比较。在这项研究中,我们使用人黑色素瘤M14野生型(WT)细胞及其耐药对应物,M14多药耐药(ADR)黑色素瘤细胞,通过长时间接触阿霉素(DOX)选择。将肿瘤细胞与人脐静脉内皮细胞(HUVEC)单层共培养。本文报道的结果表明:(i)耐药黑素瘤细胞的跨内皮迁移比敏感黑素瘤细胞更快;(ii)内皮细胞似乎受到黑素瘤细胞的迁移的强烈影响,黑素瘤细胞的迁移表现出降解其细胞质的能力;(iii)耐药细胞优先采用跨细胞侵袭细胞旁一种;(iv)肿瘤金属蛋白酶介导的内皮损伤似乎是可逆的。
Cancer cell extravasation is a crucial step in cancer metastasis. However, many of the mechanisms involved in this process are only now being elucidated. Thus, in the present
study we analysed the trans-endothelial invasion of melanoma cells by a high throughput label-free cell impedance assay applied to transwell chamber invasion assay. This technique monitors and quantifies in real-time the invasion of endothelial cells by malignant tumour cells, for a long time, avoiding artefacts due to preparation of the end point measurements. Results obtained by impedance analysis were compared with endpoint measurements. In this
study, we used human melanoma M14 wild type (WT) cells and their drug resistant counterparts, M14 multidrug resistant (ADR) melanoma cells, selected by prolonged exposure to doxorubicin (DOX). Tumour cells were co-cultured with monolayers of human umbilical vein endothelial cells (HUVEC). Results herein reported demonstrated that: (i) the trans-endothelial migration of resistant melanoma cells was faster than sensitive ones; (ii) the endothelial cells appeared to be strongly affected by the transmigration of melanoma cells which showed the ability to degrade their cytoplasm; (iii) resistant cells preferentially adopted the transcellular invasion vs. the paracellular one; (iv) the endothelial damage mediated by tumour metalloproteinases seemed to be reversible.