After the emergence of African swine fever (ASF), the wild boar population numbers fell drastically in Eastern Europe. This situation made it possible to verify the changes in the wolves\' diet that occurred. The material collection was carried out in two regions, Grodno and Vitebsk, in Belarus. In total, 19 species/groups of prey were observed in the gray wolf diet, but the most important were wild boar, elk, red deer, roe deer and beaver. The decrease in the number of wild boar caused changes in the diet of wolves but only in Vitebsk region, where wolves\' diet before the ASF epidemic outbreak consisted mainly of elk and wild boar. After the decrease of wild boar numbers, wolves still mainly hunted elk, but other types of prey included roe deer, red deer and beaver. We found a negative correlation between wild boar and both deer species (roe deer and red deer) in the wolves\' diet. Moreover, the more the wolves consumed elk, the less they consumed beaver. In our opinion, only intensive hunting of wolves by humans can explain the resulting dietary fluctuations between elk and beaver, as well as the fact that wolves did not turn to other food sources.

Beavers can profoundly alter riparian environments, most conspicuously by creating dams and wetlands. Eurasian beaver (Castor fiber) populations are increasing and it has been suggested they could play a role in the provision of multiple ecosystem services, including natural flood management. Research at different scales, in contrasting ecosystems is required to establish to what extent beavers can impact on flood regimes. Therefore, this study determines whether flow regimes and flow responses to storm events were altered following the building of beaver dams and whether a flow attenuation effect could be significantly attributed to beaver activity. Four sites were monitored where beavers have been reintroduced in England. Continuous monitoring of hydrology, before and after beaver impacts, was undertaken on streams where beavers built sequences of dams. Stream orders ranged from 2nd to 4th, in both agricultural and forest-dominated catchments. Analysis of >1000 storm events, across four sites showed an overall trend of reduced total stormflow, increased peak rainfall to peak flow lag times and reduced peak flows, all suggesting flow attenuation, following beaver impacts. Additionally, reduced high flow to low flow ratios indicated that flow regimes were overall becoming less \"flashy\" following beaver reintroduction. Statistical analysis, showed the effect of beaver to be statistically significant in reducing peak flows with estimated overall reductions in peak flows from -0.359 to -0.065 m3 s-1 across sites. Analysis showed spatial and temporal variability in the hydrological response to beaver between sites, depending on the level of impact and seasonality. Critically, the effect of beavers in reducing peak flows persists for the largest storms monitored, showing that even in wet conditions, beaver dams can attenuate average flood flows by up to ca. 60%. This research indicates that beavers could play a role in delivering natural flood management.

Giardia causes the diarrheal disease known as giardiasis; transmission through contaminated surface water is common. The protozoan parasite\'s genetic diversity has major implications for human health and epidemiology. To determine the extent of transmission from wildlife through surface water, we performed whole-genome sequencing (WGS) to characterize 89 Giardia duodenalis isolates from both outbreak and sporadic infections: 29 isolates from raw surface water, 38 from humans, and 22 from veterinary sources. Using single nucleotide variants (SNVs), combined with epidemiological data, relationships contributing to zoonotic transmission were described. Two assemblages, A and B, were identified in surface water, human, and veterinary isolates. Mixes of zoonotic assemblages A and B were seen in all the community waterborne outbreaks in British Columbia (BC), Canada, studied. Assemblage A was further subdivided into assemblages A1 and A2 based on the genetic variation observed. The A1 assemblage was highly clonal; isolates of surface water, human, and veterinary origins from Canada, United States, and New Zealand clustered together with minor variation, consistent with this being a panglobal zoonotic lineage. In contrast, assemblage B isolates were variable and consisted of several clonal lineages relating to waterborne outbreaks and geographic locations. Most human infection isolates in waterborne outbreaks clustered with isolates from surface water and beavers implicated to be outbreak sources by public health. In-depth outbreak analysis demonstrated that beavers can act as amplification hosts for human infections and can act as sources of surface water contamination. It is also known that other wild and domesticated animals, as well as humans, can be sources of waterborne giardiasis. This study demonstrates the utility of WGS in furthering our understanding of Giardia transmission dynamics at the water-human-animal interface.IMPORTANCEGiardia duodenalis causes large numbers of gastrointestinal illness in humans. Its transmission through the contaminated surface water/wildlife intersect is significant, and the water-dwelling rodents beavers have been implicated as one important reservoir. To trace human infections to their source, we used genome techniques to characterize genetic relationships among 89 Giardia isolates from surface water, humans, and animals. Our study showed the presence of two previously described genetic assemblages, A and B, with mixed infections detected from isolates collected during outbreaks. Study findings also showed that while assemblage A could be divided into A1 and A2, A1 showed little genetic variation among animal and human hosts in isolates collected from across the globe. Assemblage B, the most common type found in the study surface water samples, was shown to be highly variable. Our study demonstrates that the beaver is a possible source of human infections from contaminated surface water, while acknowledging that theirs is only one role in the complex cycle of zoonotic spread. Mixes of parasite groups have been detected in waterborne outbreaks. More information on Giardia diversity and its evolution using genomics will further the understanding of the epidemiology of spread of this disease-causing protozoan.