背景:自体脂肪移植(AFG)已成为一种备受追捧的整形外科手术,尽管它的成功受到不确定的脂肪存活率的阻碍。目前的证据表明,脂肪来源的干细胞(ADSC)可能有助于脂肪滞留在AFG。在以往的研究中,证实胸腺肽β4(Tβ4)可以增强体内脂肪存活,尽管确切的机制尚不清楚。
方法:从接受吸脂术的患者中分离ADSCs,并使其增殖,凋亡,抗凋亡,在Tβ4刺激下,使用细胞计数试剂盒-8,流式细胞术,伤口愈合试验,和实时定量PCR。还测定了与血管生成和Hippo信号相关的基因的mRNA水平。
结果:与对照组(0ng/mL)相比,Tβ4在100ng/mL(p值=0.0171)和1000ng/mL(p值=0.0054)下从第1天起显著增加ADSC增殖。此外,Tβ4组增殖相关基因的mRNA水平升高.此外,用Tβ4和凋亡诱导试剂刺激时,Tβ4增强ADSCs的抗凋亡能力(0ng/mL与1000ng/mL,p值=0.011)。至关重要的是,血管生成相关基因和Hippo通路中关键基因的mRNA表达水平受ADSCs中Tβ4的影响。
结论:Tβ4通过促进ADSC增殖和减少细胞凋亡而增强AFG的脂肪活力,并充当ADSC相关血管生成的关键正调节剂。此外,Tβ4可能通过调节Hippo途径对ADSCs进行表型调节。
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BACKGROUND: Autologous fat grafting (AFG) has emerged as a highly sought-after plastic surgery procedure, although its success has been hampered by the uncertain fat survival rate. Current evidence suggests that adipose-derived stem cells (ADSCs) may contribute to fat retention in AFG. In previous studies, it was confirmed that thymosin beta 4 (Tβ4) could enhance fat survival in vivo, although the precise mechanism remains unclear.
METHODS: ADSCs were isolated from patients undergoing liposuction and their proliferation, apoptosis, anti-apoptosis, and migration were analyzed under Tβ4 stimulation using cell counting kit-8, flow cytometry, wound healing assay, and real-time quantitative PCR. The mRNA levels of genes relating to angiogenesis and Hippo signaling were also determined.
RESULTS: Tβ4 at 100 ng/mL (p-value = 0.0171) and 1000 ng/mL (p-value = 0.0054) significantly increased ADSC proliferation from day 1 compared to the control group (0 ng/mL). In addition, the mRNA levels of proliferation-associated genes were elevated in the Tβ4 group. Furthermore, Tβ4 enhanced the anti-apoptotic ability of ADSCs when stimulated with Tβ4 and an apoptotic induction reagent (0 ng/mL vs. 1000 ng/mL, p-value = 0.011). Crucially, the mRNA expression levels of angiogenesis-related genes and critical genes in the Hippo pathway were affected by Tβ4 in ADSCs.
CONCLUSIONS: Tβ4 enhances adipose viability in AFG via facilitating ADSC proliferation and reducing apoptosis, and acts as a crucial positive regulator of ADSC-associated angiogenesis. Additionally, Tβ4 could be accountable for the phenotypic adjustment of ADSCs by regulating the Hippo pathway.
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