背景:皮肤衰老是最丰富的衰老相关疾病之一,可以通过过度暴露于紫外线照射而加速。局部施用的发酵护肤成分由于其高浓度的各种皮肤滋养营养素和生物活性成分以及低皮肤刺激效力而获得了越来越多的关注。
目的:在本研究中,我们的目标是充分展示嗜热热菌和枯草芽孢杆菌混合培养发酵(TBFE)的新型提取物的皮肤相关益处。
方法:通过创新的混合培养发酵工艺制备TBFE。相应地通过不同的方法定量营养素和生物活性成分的含量。体外试验和随机对照人体试验均用于进一步证明对人体皮肤的多方面有益作用。以及潜在的机制。
结果:我们的结果显示TBFE上调IV型胶原的表达,弹性蛋白,水通道蛋白-3和真皮-表皮连接标记,同时抑制黑色素的产生,在不同的皮肤细胞模型中。此外,TBFE抑制正常人角质形成细胞中紫外线照射诱导的活性氧和促炎介质的产生,同时刺激衰老角质形成细胞的自噬。临床研究的结果证实了这些体外发现,证明TBFE在5%和20%浓度下可在皮肤敏感的受试者中提供抗衰老特性,在改善皱纹方面,保湿,和皮肤美白。
结论:总之,我们证明了一种新型的混合培养发酵提取物具有有希望的抗衰老作用,这可能归因于抗氧化,抗炎,促进皮肤细胞的自噬。
BACKGROUND: Skin aging is one of the most abundant aging-related disorders that can be accelerated by excessive exposure to ultraviolet irradiation. Topically applied fermented skincare ingredients have gained mounting attentions due to their high concentration of various skin nourishing nutrients and bioactive components and low skin irritation potency.
OBJECTIVE: In the present study, we aim to fully demonstrate the skin-related benefits of a novel extract of Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE).
METHODS: TBFE was prepared through an innovative mixed-culture fermentation process. The contents of nutrients and bioactive ingredients were quantified by different methods accordingly. Both in vitro tests and randomized controlled human trial were utilized to further demonstrate multifaceted beneficial effects on human skin, as well as the potential mechanisms.
RESULTS: Our results showed that TBFE upregulated the expression of type IV collagen, elastin, aquaporin-3, and dermal-epidermal junction markers, while inhibited production of melanin, in different skin cell models. Moreover, TBFE inhibited the generation of reactive oxygen species and pro-inflammatory mediators induced by ultraviolet irradiation in normal human keratinocytes, while stimulated autophagy in senescent keratinocytes. Results from clinical studies confirmed those in vitro findings, demonstrating that TBFE at 5% and 20% concentration provides anti-aging properties in subjects with sensitive skin, in terms of improving wrinkles, moisturization, and skin lightening.
CONCLUSIONS: In summary, we demonstrate that a novel mixed-culture ferment extract has promising anti-aging effects, which may be attributed to anti-oxidation, anti-inflammation, and promotion of autophagy in skin cells.