细胞行为,真核生物的细胞分化和个体发育是表观遗传和经典分子遗传机制之间复杂相互作用的结果,其中许多相互作用仍有待阐明。组蛋白去乙酰化酶(HDAC)通过压缩核小体促进组蛋白与DNA的相互作用,从而导致转录抑制。MADS域转录因子在真核生物中高度保守,参与控制动物和植物的不同发育过程。以及调节植物的应激反应。在这项工作中,我们专注于发现拟南芥HDACs和MADS结构域蛋白的推定相互作用,使用进化的观点结合生物信息学分析,并通过经典的分子生物学工具测试更有希望的预测相互作用。通过生物信息学分析,我们发现了来自不同生物体的HDAC蛋白之间的相似性,这使我们能够预测拟南芥脱乙酰酶HDA15与MADS结构域蛋白XAANTAL1(XAL1)之间的推定蛋白质-蛋白质相互作用。双杂交和双分子荧光互补分析的结果证明了核中的体外和体内HDA15-XAL1相互作用。很可能,这种相互作用可能会调节植物的发育过程,就像动物中这种相互作用一样。
Cellular behavior, cell differentiation and ontogenetic development in eukaryotes result from complex interactions between epigenetic and classic molecular genetic mechanisms, with many of these interactions still to be elucidated. Histone deacetylase enzymes (HDACs) promote the interaction of histones with DNA by compacting the nucleosome, thus causing transcriptional repression. MADS-domain transcription factors are highly conserved in eukaryotes and participate in controlling diverse developmental processes in animals and plants, as well as regulating stress responses in plants. In this work, we focused on finding out putative interactions of Arabidopsis thaliana HDACs and MADS-domain proteins using an evolutionary perspective combined with bioinformatics analyses and testing the more promising predicted interactions through classic molecular biology tools. Through bioinformatic analyses, we found similarities between HDACs proteins from different organisms, which allowed us to predict a putative protein-protein interaction between the Arabidopsis thaliana deacetylase HDA15 and the MADS-domain protein XAANTAL1 (
XAL1). The results of two-hybrid and Bimolecular Fluorescence Complementation analysis demonstrated in vitro and in vivo HDA15-
XAL1 interaction in the nucleus. Likely, this interaction might regulate developmental processes in plants as is the
case for this type of interaction in animals.