已经提出上皮-间质转化(EMT)是几种疾病的发病机理。然而,EMT过程与牙周炎严重程度之间的关系尚未得到研究.
本研究旨在定位和定量评估转化生长因子-β1(TGF-β1)的表达,vimentin和E-cadherin与牙周病患者牙龈的EMT过程相关,与halthy个体相比。
36名参与者的牙龈组织样本分为2组:健康(对照组)组(n=9)和牙周炎组(n=27)。牙周炎组进一步细分为轻度,中度和重度牙周炎亚组(每个亚组9例)。对样品进行组织学染色,TGF-β的组织形态计量学分析和定量实时聚合酶链反应(RT-PCR)分析,波形蛋白和E-catherin.进行统计学和相关性分析。
来自中度和重度牙周炎亚组的苏木精和伊红(H&E)染色切片显示上皮增生,与对照组相比,核周卤化和炎性细胞计数显着增加。在重度牙周炎亚组中,TGF-β1和波形蛋白的平均表达值最高,而最低的平均值记录在对照牙龈中。相反,E-catherin的表达在对照牙龈中平均值最高,而平均值最低的是严重牙周炎亚组。发现所有结果均具有统计学意义。相关性分析显示牙周炎的严重程度与TGF-β和波形蛋白的表达呈统计学正相关,而E-catherin的表达与牙周炎的严重程度之间存在统计学上显着的负相关。
牙周炎的严重程度与EMT过程标志物(TGF-β和波形蛋白)的表达之间存在直接相关性。这种相关性表明EMT在牙周病的发病和预后中起重要作用。这项研究中提供的数据可以为使用抗EMT药物治疗牙周病打开大门。
It has been proposed that epithelial-mesenchymal transition (EMT) is responsible for the pathogenesis of several diseases. However, the relationship between the EMT process and the severity of periodontitis has not been previously investigated.
This study aimed to localize and quantitatively assess the expression of transforming growth factor-beta 1 (TGF-β1), vimentin and E-cadherin in correlation with the EMT process in human gingiva of periodontally diseased patients in comparison with halthy individuals.
Gingival tissue samples from 36 participants were divided into 2 groups: the healthy (control) group (n = 9); and the periodontitis group (n = 27). The periodontitis group was further subclassified into mild, moderate and severe periodontitis subgroups (9 patients in each subgroup). The samples were subjected to histological staining, the histomorphometric analysis and the quantitative real-time polymerase chain reaction (RT‑PCR) analysis for TGF-β, vimentin and E-catherin. Statistical and correlation analyses were performed.
The hematoxylin and eosin (H&E) stain sections from both the moderate and severe periodontitis subgroups showed epithelial hyperplasia, perinuclear haloing and a marked increase in the inflammatory cell count as compared to the control group. The highest mean TGF-β1 and vimentin expression values were recorded in the severe periodontitis subgroup, whereas the lowest mean values were recorded in the control gingiva. On the contrary, the expression of E-catherin had the highest mean value in the control gingiva, whereas the lowest mean value was recorded in the severe periodontitis subgroup. All results were found to be statistically significant. The correlation analysis revealed a statistically significant positive correlation between the severity of periodontitis and the expression of TGF-β and vimentin, while a statistically significant inverse correlation was found between the expression of E-catherin and the severity of periodontitis.
There is a direct correlation between the severity of periodontitis and the expression of the EMT process markers (TGF-β and vimentin). This correlation indicates that EMT plays an important role in the pathogenesis and prognosis of periodontal disease. The data presented in this study could open the door for using anti-EMT agents in treating periodontal disease.