反刍动物肠道菌群是影响动物生产和健康的重要因素。研究反刍动物肠道菌群与肉质的关联机制,对了解反刍动物肉质的形成机制,提高生产效率具有积极作用。在这项研究中,脂肪酸组成和含量,相关基因的表达,和不同年龄藏绵羊母羊回肠微生物区系的结构特征(4个月,1.5年,3.5年,和6年)进行检测和分析。结果表明,不同年龄藏绵羊肌肉脂肪酸组成和含量存在显著差异(p<0.05);背最长肌和腿肌中MUFA的含量较高。同样,肌肉相关基因的表达在不同年龄组之间存在差异,以及LPL的表达式,SCD,1.5岁组的FABP4基因更高。1.5岁组的回肠微生物群多样性较高,1.5岁组的Romboutsia丰度比率显着升高(p<0.05),与油酸(C18:1n9c)呈显著正相关(p<0.05)。总之,1.5岁时,藏绵羊背最长肌和腿部肌肉中有益脂肪酸含量较高,最佳屠宰年龄为1.5岁。本研究为深入研究肠道菌群对肉品质的影响机制及相关调控提供了参考。
The intestinal microbiota of ruminants is an important factor affecting animal production and health. Research on the association mechanism between the intestinal microbiota and meat quality of ruminants will play a positive role in understanding the formation mechanism of meat quality in ruminants and improving production efficiency. In this
study, the fatty acid composition and content, expression of related genes, and structural characteristics of the ileum microbiota of ewes of Tibetan sheep at different ages (4 months, 1.5 years, 3.5 years, and 6 years) were detected and analyzed. The results revealed significant differences in fatty acid composition and content in the muscle of Tibetan sheep at different ages (p < 0.05); in addition, the content of MUFAs in the longissimus dorsi muscle and leg muscle was higher. Similarly, the expressions of muscle-related genes differed among the different age groups, and the expression of the LPL, SCD, and FABP4 genes was higher in the 1.5-year-old group. The ileum microbiota diversity was higher in the 1.5-year-old group, the Romboutsia abundance ratio was significantly higher in the 1.5-year-old group (p < 0.05), and there was a significant positive correlation with oleic acid (C18:1n9c) (p < 0.05). In conclusion, the content of beneficial fatty acids in the longissimus dorsi muscle and leg muscle of Tibetan sheep was higher at 1.5 years of age, and the best slaughter age was 1.5 years. This
study provides a reference for in-depth research on the mechanism of the influence of the gut microbiota on meat quality and related regulation.