养殖业生产实践的加强,导致滥用抗生素防治疾病,降低成本,造成了环境污染,对水产养殖的可持续性和食品安全构成严重威胁。然而,氟苯尼考(FF)对甲壳类动物肝胰腺的毒性作用尚不清楚。在这里,通过使用中华绒螯蟹(Eriocheirsinensis)作为研究对象,研究对组织病理学的毒性作用,氧化应激,与环境相关(0.5和5μg/L)的肝胰腺细胞凋亡和微生物群,和极端浓度(50μg/L)的FF。我们的结果表明,FF暴露以剂量和时间依赖性方式引起的肝胰腺组织结构损伤。结合凋亡相关基因(Caspase3、Caspase8、p53、Bax和Bcl-2)在mRNA和蛋白水平的表达增加,活化过氧化氢酶(CAT)和超氧化物歧化酶(SOD),丙二醛(MDA)积累,FF暴露也会引起氧化应激,肝胰腺细胞凋亡。有趣的是,在与环境相关的FF浓度下,7天的暴露对螃蟹的毒性作用比14天更明显。综合生物标志物反应版本2(IBRv2)指数表明,极端浓度下14天FF暴露对螃蟹有严重的毒性作用。此外,暴露于FF14天改变了肝胰腺微生物群的多样性和组成,导致暴露于50μg/LFF后病原微生物螺旋藻的显着增加。一起来看,我们的研究解释了FF对甲壳类动物肝胰腺毒性的潜在机制,为FF在中华绒螯蟹养殖中的应用提供了参考。
The intensification of production practices in the aquaculture industry has led to the indiscriminate use of antibiotics to combat diseases and reduce costs, which has resulted in environmental pollution, posing serious threats to aquaculture sustainability and food safety. However, the toxic effect of florfenicol (FF) exposure on the hepatopancreas of crustaceans remains unclear. Herein, by employing Chinese mitten crab (Eriocheir sinensis) as subjects to investigate the toxic effects on histopathology, oxidative stress, apoptosis and microbiota of hepatopancreas under environment-relevant (0.5 and 5 μg/L), and extreme concentrations (50 μg/L) of FF. Our results revealed that the damage of hepatopancreas tissue structure caused by FF exposure in a dose-and time-dependent manner. Combined with the increased expression of apoptosis-related genes (Caspase 3, Caspase 8, p53, Bax and Bcl-2) at mRNA and protein levels, activation of catalase (CAT) and superoxide dismutase (SOD), and malondialdehyde (MDA) accumulation, FF exposure also induced oxidative stress, and apoptosis in hepatopancreas. Interestingly, 7 days exposure triggered more pronounced toxic effect in crabs than 14 days under environment-relevant FF concentration. Integrated biomarker response version 2 (IBRv2) index indicated that 14 days FF exposure under extreme concentration has serious toxicity effect on crabs. Furthermore, 14 days exposure to FF changed the diversity and composition of hepatopancreas microbiota leading remarkable increase of pathogenic microorganism Spirochaetes following exposure to 50 μg/L of FF. Taken together, our study explained potential mechanism of FF toxicity on hepatopancreas of crustaceans, and provided a reference for the concentration of FF to be used in culture of Chinese mitten crab.