约束和底物拓扑强烈影响细胞群的行为,特别是,他们的集体迁徙。处理这些方面的体外实验需要长时间(通常几天)保持有效的表面图案化策略以及在三维中控制表面拓扑的方法。这里,我们描述了解决这两个方面的协议。通过直接在涂覆表面上图案化的光致抗蚀剂掩模蚀刻涂层来实现稳健的细胞排斥涂层的高分辨率图案化。平面外曲率可使用玻璃丝或波纹“波浪形”曲面进行控制。
Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated \"wavy\" surfaces.