背景:人芽囊原虫(B.hominis)是一种全球分布的原生动物寄生虫。一些研究表明,人芽孢杆菌与肠易激综合征(IBS)的发展之间存在联系。这项研究的目的是确定与健康个体相比,IBS患者中人源双歧杆菌的患病率。
方法:共检查了来自伊朗北部IBS患者的65个粪便样本和来自健康个体的65个样本。使用各种方法测试样品,包括直接涂片,福尔马林醚沉降培养检测人源芽孢杆菌的存在。此外,对所有培养阳性分离株进行聚合酶链反应(PCR)以确认结果并鉴定基因型.
结果:B.15.38%的IBS患者和9.2%的健康人群中检测到了人源病。发现RPMI1640中的培养物比福尔马林醚和直接涂片方法更好。使用分子方法确认阳性样品。两组之间的人源芽孢杆菌感染顺序没有显着差异。
结论:我们的研究结果表明,在IBS患者和健康组之间,人源芽孢杆菌感染的顺序没有显着差异。因此,需要进一步研究以确定该寄生虫的潜在致病作用及其在引起IBS中的作用。
BACKGROUND: Blastocystis hominis (B. hominis) is a protozoan parasite that has a worldwide distribution. Some studies have suggested a link between B. hominis and the development of irritable bowel syndrome (IBS). The objective of this study was to determine the prevalence of B. hominis in patients with IBS compared to healthy individuals.
METHODS: A total of 65 stool samples from patients with IBS and 65 samples from healthy individuals in northern Iran were examined. The samples were tested using various methods including direct smear, formalin ether sedimentation and culture to detect the presence of B. hominis. Additionally, polymerase chain reaction (PCR) was performed on all culture-positive isolates to confirm the results and identify the genotype.
RESULTS: B. hominis was detected in 15.38% of IBS patients and 9.2% of the healthy group. The culture in RPMI1640 was found to be better than the formalin ether and direct smear methods. Positive samples were confirmed using the molecular method. No significant difference was observed in the order of B. hominis infection between the two groups.
CONCLUSIONS: The results of our study indicate that no significant difference was observed in the order of B. hominis infection between IBS patients and healthy groups. Therefore, further study is necessary to determine the potential pathogenic effects of this parasite and its role in causing IBS.