这项研究检查了绿僵菌(Hypecreales:Clavipitaceae)分生孢子对卵的影响,幼虫,蛹,和成虫的节食夜蛾.结果表明,鸡蛋,幼虫,蛹,成年人的死亡率取决于剂量。发现分生孢子的数量增加(1.5×109分生孢子/mL)对幼虫有毒,蛹,和成人治疗9天后,导致100%的鸡蛋死亡率,98%的幼虫,76%的蛹,85%的成年人。一项使用蚯蚓作为生物指标的研究发现,暴露3天后,双歧杆菌分生孢子对蚯蚓没有任何有害影响。相比之下,化学处理(阳性对照)在40ppm的浓度下导致100%的死亡率。组织病理学研究表明,与阴性对照相比,用真菌分生孢子处理的蚯蚓肠组织没有显着差异。用久效磷处理的蚯蚓的肠道组织表现出明显的损伤,在化学处理中观察到显著差异。用70和100μg/mL的Eudrilus表皮粘液溶液处理没有显示真菌生长。在生化水平上对酶的分析显示乙酰胆碱酯酶水平降低,α-羧酸酯酶,和β-羧酸酯酶在暴露于真菌分生孢子后。这项研究发现,马尾藻对S.frugiperda有效,强调这种昆虫病原真菌在控制这种农业害虫方面的潜力。
This
study examined the impact of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) conidia on the eggs, larvae, pupae, and adults of Spodoptera frugiperda. The results showed that eggs, larvae, pupae, and adults exhibited mortality rates that were dependent on the dose. An increased amount of conidia (1.5 × 109 conidia/mL) was found to be toxic to larvae, pupae, and adults after 9 days of treatment, resulting in a 100% mortality rate in eggs, 98% in larvae, 76% in pupae, and 85% in adults. A
study using earthworms as bioindicators found that after 3 days of exposure, M. anisopliae conidia did not cause any harmful effects on the earthworms. In contrast, the chemical treatment (positive control) resulted in 100% mortality at a concentration of 40 ppm. Histopathological studies showed that earthworm gut tissues treated with fungal conidia did not show significant differences compared with those of the negative control. The gut tissues of earthworms treated with monocrotophos exhibited significant damage, and notable differences were observed in the chemical treatment. The treatments with 70 and 100 µg/mL solutions of Eudrilus eugeniae epidermal mucus showed no fungal growth. An analysis of the enzymes at a biochemical level revealed a decrease in the levels of acetylcholinesterase, α-carboxylesterase, and β-carboxylesterase in S. frugiperda larvae after exposure to fungal conidia. This
study found that M. anisopliae is effective against S. frugiperda, highlighting the potential of this entomopathogenic fungus in controlling this agricultural insect pest.