本案例研究旨在帮助学生探索剪接体的分子机制以及SARS-CoV-2如何影响宿主细胞剪接体功能,同时解释主要文献中的数字(A.K.Banjeree,etal.,单元183:1325-1339,e1-e10,2020年,https://doi.org/10.1016/j。cell.2020.10.004)。“皮特和失踪的剪刀”是在2022年春季和2022年秋季在两个大型招生(150+)分子生物学入门课程中设计和实施的,公共研究机构。案例研究的格式与国家科学案例研究教学中心(NCCSTS)框架保持一致,这已经被证明是有效的,以学生为中心的方法在本科阶段教授复杂的生物学概念。该案例研究有四个学生学习目标(SLO),与Bloom的修订分类法保持一致,并要求学生对剪接的分子机制有一个理解,并分析和解释主要文献中的一个数字。本活动包括形成性和总结性评估问题,每个问题映射到一个案例研究SLO。总结性评估问题以前/后的方式给出,配对t检验用于评估学生评估前后分数之间的差异。评估结果表明,两个课程的学生都掌握了本案例研究的每个SLO,鉴于与评估前相比,评估后分数显着增加。这些发现表明,“皮特和缺失的剪刀”案例研究是培养学生对剪接体理解的有效方法,以及从主要文献中解读数字的能力。
This case
study was designed to help students explore the molecular mechanisms of the
spliceosome and how SARS-CoV-2 impacts host cell spliceosomal function while interpreting figures from primary literature (A. K. Banjeree, et al., Cell 183:1325-1339, e1-e10, 2020, https://doi.org/10.1016/j.cell.2020.10.004). \"Pete and the Missing Scissors\" was designed and implemented in the spring of 2022 and fall of 2022 in two large-enrollment (150+) introductory molecular biology courses at a large, public research institution. The case
study was formatted in alignment with the National Center for Case
Study Teaching in Science (NCCSTS) framework, which has been shown to be an effective, student-centered approach to teaching complex biological concepts at the undergraduate level. The case
study had four student learning objectives (SLOs) that aligned with Bloom\'s Revised Taxonomy and required students to develop an understanding of the molecular mechanisms of splicing and analyze and interpret a figure from primary literature. Both formative and summative assessment questions are included in this activity, with each question mapping to one of the case study SLOs. Summative assessment questions were given in a pre-/post-manner, and a paired t-test was used to evaluate differences between students\' pre- and post-assessment scores. Assessment results demonstrated that students in both courses mastered each of the SLOs of this case study, given the significant increase in post-assessment scores compared to the pre-assessment. These findings indicate that the \"Pete and the Missing Scissors\" case
study is an effective approach to develop students\' understanding of the
spliceosome, as well as ability to interpret figures from primary literature.