背景:患有多囊卵巢综合征(PCOS)的女性具有较高的肠粘膜通透性,导致脂多糖(LPS)渗漏和内毒素血症。这个,反过来,导致由肠-脑轴引起的氧化应激(OS)和神经炎症,影响脑源性神经营养因子(BDNF)和S100钙结合蛋白B(S100B)水平等神经营养因子水平。在这项研究中,据推测,菠菜补充的类囊体膜以及低热量饮食可能改善了LPS水平,神经营养因子,PCOS患者的OS。
方法:在这种双盲中,随机化,安慰剂对照,和临床试验,48名根据鹿特丹标准诊断为PCOS的肥胖女性被随机分为类囊体组(N=21)和安慰剂组(N=23)。为所有患者规定了比总能量消耗少500卡路里的个性化低热量饮食。参与者每天补充5克/天富含类囊体的菠菜提取物或安慰剂(5克玉米淀粉),持续12周,并规定低热量饮食。在基线和12周干预后评估人体测量和生化参数。
结果:在接受口服类囊体补充剂和低热量饮食的参与者中,LPS水平显著下降(P<0.001)和BDNF水平升高(P<0.001)。此外,空腹血糖显着下降,胰岛素,胰岛素抵抗的稳态评估模型,游离睾酮指数,两组卵泡刺激素/黄体生成素比值(P<0.05)。两组间丙二醛变化无显著差异,过氧化氢酶,总抗氧化能力,和S100B水平(P>0.05)。
结论:总而言之,补充低热量饮食的菠菜类囊体膜降低了LPS水平,增加BDNF水平,并改善了血糖状况和性激素水平;然而,干预12周后,他们对OS标志物水平无影响.
BACKGROUND: Women with polycystic ovary syndrome (PCOS) have higher intestinal mucosal permeability, leading to the lipopolysaccharide (LPS) leakage and endotoxemia. This, in turn, leads to oxidative stress (OS) and neuro-inflammation caused by the gut-brain axis, affecting the neurotrophic factors levels such as brain-derived neurotrophic factor (BDNF) and S100 calcium-binding protein B (S100 B) levels. In this
study, it was hypothesized that the thylakoid membranes of spinach supplementation along with a hypocaloric diet may have improved the LPS levels, neurotrophic factors, and OS in PCOS patients.
METHODS: In this double-blind, randomized, placebo-controlled, and clinical
trial, 48 women with obesity and diagnosed with PCOS based on Rotterdam criteria were randomly assigned to thylakoid (N = 21) and placebo groups (N = 23). A personalized hypocaloric diet with 500 calories less than the total energy expenditure was prescribed to all patients. The participants were daily supplemented with either a 5 g/day thylakoid-rich spinach extract or a placebo (5 g cornstarch) for 12 weeks along with a prescribed low-calorie diet. Anthropometric measurements and biochemical parameters were assessed at baseline and after the 12-week intervention.
RESULTS: A statistically significant decrease in the LPS levels (P < 0.001) and an increase in the BDNF levels (P < 0.001) were recorded for the participants receiving the oral thylakoid supplements and a low-calorie diet. Furthermore, significant decreases were observed in fasting blood glucose, insulin, homeostatic model of assessment for insulin resistance, free testosterone index, and follicle-stimulating hormone / luteinizing hormone ratio in both groups (P < 0.05). No significant differences were detected between the two groups regarding the changes in malondialdehyde, catalase, total antioxidant capacity, and S100B levels (P > 0.05).
CONCLUSIONS: In sum, the thylakoid membranes of spinach supplemented with a hypocaloric diet reduced the LPS levels, increased the BDNF levels, and improved the glycemic profile and sex-hormone levels; however, they had no effects on the OS markers levels after 12 weeks of intervention.