Spectrophotometry, Infrared

分光光度法,红外线
  • 文章类型: Journal Article
    早期发现妇科癌症,这对提高患者生存率至关重要,由于模糊的早期症状和当前方法的诊断局限性,因此具有挑战性。这篇全面的综述深入探讨了红外(IR)光谱技术改变游戏规则的潜力,用于改变妇科癌症诊断领域的非侵入性技术。通过收集组织样本内化学键的独特振动频率,傅里叶变换红外(FTIR)光谱提供了优于现有诊断方法的“分子指纹”。我们强调这一领域的重大进展,特别是在中近红外光谱中的离散生物标记带的识别。蛋白质,脂质,碳水化合物,和核酸表现出不同的吸收模式。这些光谱特征不仅有助于区分恶性和良性疾病,但也提供了有关与癌症相关的细胞变化的额外信息。为了强调这些发现的实际后果,我们检查了红外光谱显示出卓越诊断准确性的研究.这篇综述支持红外光谱在正常临床实践中的使用,强调其检测和理解妇科癌症复杂分子基础的能力。
    The early detection of gynecological cancers, which is critical for improving patient survival rates, is challenging because of the vague early symptoms and the diagnostic limitations of current approaches. This comprehensive review delves into the game-changing potential of infrared (IR) spectroscopy, a noninvasive technology used to transform the landscape of cancer diagnosis in gynecology. By collecting the distinctive vibrational frequencies of chemical bonds inside tissue samples, Fourier-transform infrared (FTIR) spectroscopy provides a \'molecular fingerprint\' that outperforms existing diagnostic approaches. We highlight significant advances in this field, particularly the identification of discrete biomarker bands in the mid- and near-IR spectra. Proteins, lipids, carbohydrates, and nucleic acids exhibited different absorption patterns. These spectral signatures not only serve to distinguish between malignant and benign diseases, but also provide additional information regarding the cellular changes associated with cancer. To underscore the practical consequences of these findings, we examined studies in which IR spectroscopy demonstrated exceptional diagnostic accuracy. This review supports the use of IR spectroscopy in normal clinical practice, emphasizing its capacity to detect and comprehend the intricate molecular underpinnings of gynecological cancers.
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  • 文章类型: Journal Article
    分子振动在物理化学和生物化学中起着至关重要的作用,拉曼和红外光谱是振动光谱中最常用的两种技术。这些技术提供了样品中分子的独特指纹,可以用来识别化学键,功能组,和分子的结构。在这篇评论文章中,讨论了使用拉曼和红外光谱进行分子指纹检测的最新研究和开发活动,专注于识别特定的生物分子和研究用于癌症诊断应用的生物样品的化学成分。还讨论了每种技术的工作原理和仪器,以更好地理解振动光谱学的分析多功能性。拉曼光谱是研究分子及其相互作用的宝贵工具,它的使用在未来可能会继续增长。研究表明,拉曼光谱能够准确诊断各种类型的癌症,使其成为内窥镜等传统诊断方法的有价值的替代方法。红外光谱可以为拉曼光谱提供补充信息,并检测低浓度的多种生物分子,甚至在复杂的生物样本中。本文最后比较了技术和对未来方向的见解。
    Molecular vibrations play a crucial role in physical chemistry and biochemistry, and Raman and infrared spectroscopy are the two most used techniques for vibrational spectroscopy. These techniques provide unique fingerprints of the molecules in a sample, which can be used to identify the chemical bonds, functional groups, and structures of the molecules. In this review article, recent research and development activities for molecular fingerprint detection using Raman and infrared spectroscopy are discussed, with a focus on identifying specific biomolecules and studying the chemical composition of biological samples for cancer diagnosis applications. The working principle and instrumentation of each technique are also discussed for a better understanding of the analytical versatility of vibrational spectroscopy. Raman spectroscopy is an invaluable tool for studying molecules and their interactions, and its use is likely to continue to grow in the future. Research has demonstrated that Raman spectroscopy is capable of accurately diagnosing various types of cancer, making it a valuable alternative to traditional diagnostic methods such as endoscopy. Infrared spectroscopy can provide complementary information to Raman spectroscopy and detect a wide range of biomolecules at low concentrations, even in complex biological samples. The article concludes with a comparison of the techniques and insights into future directions.
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  • 文章类型: Journal Article
    红外光谱(波长范围为750-25,000nm)提供了评估各种样品类型的化学成分的快速方法,用于定性和定量分析。在过去的五十年中,其在食品工业中的使用显着增加,现在已成为常规分析某些分析物的公认分析技术。此外,它通常用于许多行业环境中的常规筛选和质量控制目的,虽然不是典型的生物活性化合物的分析。使用Scopus数据库,对2016年至2020年5年的文献进行了系统检索,确定了45项使用近红外和17项使用中红外光谱对食品中生物活性化合物进行定量的研究.评估的最常见的生物活性化合物是多酚,花青素,类胡萝卜素和抗坏血酸。许多因素影响所开发模型的准确性,包括分析物类别和浓度,矩阵类型,仪器几何,波长选择和光谱处理/预处理方法。此外,只有少数研究在独立来源的样本上进行了验证.然而,结果表明,红外光谱技术有望快速评估食品基质中各种生物活性化合物。
    Infrared spectroscopy (wavelengths ranging from 750-25,000 nm) offers a rapid means of assessing the chemical composition of a wide range of sample types, both for qualitative and quantitative analyses. Its use in the food industry has increased significantly over the past five decades and it is now an accepted analytical technique for the routine analysis of certain analytes. Furthermore, it is commonly used for routine screening and quality control purposes in numerous industry settings, albeit not typically for the analysis of bioactive compounds. Using the Scopus database, a systematic search of literature of the five years between 2016 and 2020 identified 45 studies using near-infrared and 17 studies using mid-infrared spectroscopy for the quantification of bioactive compounds in food products. The most common bioactive compounds assessed were polyphenols, anthocyanins, carotenoids and ascorbic acid. Numerous factors affect the accuracy of the developed model, including the analyte class and concentration, matrix type, instrument geometry, wavelength selection and spectral processing/pre-processing methods. Additionally, only a few studies were validated on independently sourced samples. Nevertheless, the results demonstrate some promise of infrared spectroscopy for the rapid estimation of a wide range of bioactive compounds in food matrices.
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  • 文章类型: Journal Article
    许多危及生命的疾病在其早期疾病阶段仍然不清楚。症状只出现在晚期时,存活率很低。即使在渐近阶段,非侵入性诊断工具也可能能够识别疾病并挽救生命。基于挥发性代谢物的诊断具有满足这一需求的很大希望。正在开发许多实验技术来建立可靠的非侵入性诊断工具;然而,他们中还没有一个能够满足临床医生的要求。基于红外光谱的气态生物流体分析显示了有希望的结果,可以满足临床医生的期望。标准操作程序(SOP)的最新发展,样品测量,本文综述了红外光谱的数据分析技术。它还概述了红外光谱的适用性,以确定特定的生物标志物的疾病,如糖尿病,由细菌感染引起的急性胃炎,脑瘫,和前列腺癌。
    Many life-threatening diseases remain obscure in their early disease stages. Symptoms appear only at the advanced stage when the survival rate is poor. A non-invasive diagnostic tool may be able to identify disease even at the asymptotic stage and save lives. Volatile metabolites-based diagnostics hold a lot of promise to fulfil this demand. Many experimental techniques are being developed to establish a reliable non-invasive diagnostic tool; however, none of them are yet able to fulfil clinicians\' demands. Infrared spectroscopy-based gaseous biofluid analysis demonstrated promising results to fulfil clinicians\' expectations. The recent development of the standard operating procedure (SOP), sample measurement, and data analysis techniques for infrared spectroscopy are summarized in this review article. It has also outlined the applicability of infrared spectroscopy to identify the specific biomarkers for diseases such as diabetes, acute gastritis caused by bacterial infection, cerebral palsy, and prostate cancer.
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  • 文章类型: Journal Article
    物质的红外光谱分析是一种非侵入性测量技术,可用于分析。尽管这项研究的主要目的是提供对机器学习(ML)算法的回顾,这些算法已被报道用于分析从传统机器学习方法到深度网络体系结构的近红外(NIR)光谱,我们还提供不同的近红外测量模式,仪器,信号预处理方法,等。首先,审查了NIR中可用的四种不同的测量模式,比较了不同类型的NIR仪器,并对近红外数据分析方法进行了总结。其次,简要讨论了公共近红外光谱数据集,提供链接。第三,介绍了已报道的用于近红外光谱的广泛使用的数据预处理和特征选择算法。然后,覆盖了大多数传统的机器学习方法和常用的深度网络架构。最后,我们得出的结论是,以高效和轻量级的方式开发多种机器学习算法的集成是一个重要的未来研究方向。
    The analysis of infrared spectroscopy of substances is a non-invasive measurement technique that can be used in analytics. Although the main objective of this study is to provide a review of machine learning (ML) algorithms that have been reported for analyzing near-infrared (NIR) spectroscopy from traditional machine learning methods to deep network architectures, we also provide different NIR measurement modes, instruments, signal preprocessing methods, etc. Firstly, four different measurement modes available in NIR are reviewed, different types of NIR instruments are compared, and a summary of NIR data analysis methods is provided. Secondly, the public NIR spectroscopy datasets are briefly discussed, with links provided. Thirdly, the widely used data preprocessing and feature selection algorithms that have been reported for NIR spectroscopy are presented. Then, the majority of the traditional machine learning methods and deep network architectures that are commonly employed are covered. Finally, we conclude that developing the integration of a variety of machine learning algorithms in an efficient and lightweight manner is a significant future research direction.
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  • 文章类型: Journal Article
    本研究旨在通过FTIR收集有关土壤调查的信息。我们知道,FTIR技术最常用于有机和生物有机化学,而在地球化学中,FTIR光谱并不经常使用。因此,沉积物和土壤中所含矿物的红外光谱的识别和解释存在问题。其原因是缺乏有关矿物特征波数的数据。因此,本研究进行了回顾和总结,在一个地方,发表的文章与2002年至2021年的矿物调查有关(基于Scopus数据库)。此外,本综述重点介绍了各种分析技术(ATR-FTIR,漂移,2D-IR,和SR-FTIR),并讨论了其中的一些用于地球化学研究。此外,这项研究描述了红外光谱数据预处理中的有用工具(归一化,基线校正,和光谱导数)。
    This study aims to collect information about soil investigation by FTIR. As we know, the FTIR technique is most often used in organic and bioorganic chemistry, while in geochemistry FTIR spectroscopy is not used very often. Therefore, there is a problem with the identification and interpretation of the IR spectra of minerals contained in sediments and soils. The reason for this is a deficiency of data about characteristic wavenumbers for minerals. Therefore, this study reviews and sums up, in one place, published articles that are connected to an investigation of minerals from 2002 to 2021 (based on the Scopus database). Additionally, the present review highlights various analytical techniques (ATR-FTIR, DRIFT, 2D-IR, and SR-FTIR) and discusses some of them for geochemical study. Additionally, the study describes helpful tools in the data pre-processing of IR spectra (normalization, baseline correction, and spectral derivatives).
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  • 文章类型: Journal Article
    如今,野生食用牛肝菌由于其天然健康而在消费者中越来越有吸引力,营养,和美味的特点。这些食用蘑菇的质量控制和评估需要适当的分析技术以及多元统计分析。紫外可见(UV-Vis)和红外(IR)技术具有省时、低成本,环保,现在是主要的分析技术中突出的质量评价的bolete蘑菇。已开发出化学计量学方法,以结合光谱解决牛肝菌的分类和回归问题。本文综述了UV-Vis和IR技术结合化学计量学在野生食用香菇中的最新应用。包括物种的鉴定,origin,和存储持续时间,欺诈检测,和抗氧化性能评估,并讨论了光谱学技术在蘑菇研究中的局限性和前景,以期为野生食用香菇的进一步研究和实际应用提供参考。
    Nowadays, wild edible bolete mushrooms are more and more attractive among consumers due to their natural health, nutrition, and delicious characteristics. Appropriate analytical techniques together with multivariate statistics analysis are required for the quality control and evaluation of these edible mushrooms. Ultraviolet-visible (UV-Vis) and infrared (IR) technologies have the advantages of time-saving, low-cost, and environmentally friendly, are now prominent among major analytical technologies for quality evaluation of bolete mushrooms. Chemometrics methods have been developed to solve classification and regression issues of bolete mushrooms in combination with spectrum. This paper reviewed the most recent applications of UV-Vis and IR technology coupled with chemometrics in wild edible bolete mushrooms, including the identification of species, origin, and storage duration, fraud detection, and antioxidant properties evaluation, and discussed the limitations and prospects of spectroscopy technologies in the researches of bolete mushrooms, excepting to provide a reference for further research and practical application of wild edible bolete mushrooms.
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  • 文章类型: Journal Article
    在全球范围内,葡萄酒交易涉及可观的经济价值,保证葡萄酒真实性的要求永远不可低估。随着分析平台的不断发展,光谱方法的研究正在蓬勃发展,因为它们为快速葡萄酒认证提供了强大的工具。特别是,光谱技术已被确定为一种用户友好和经济的替代传统分析涉及更复杂的仪器,可能不容易部署在行业设置。化学计量学在光谱数据的解释和建模中起着不可或缺的作用,并且经常与光谱学一起用于样品分类。考虑到光谱学旗帜下的各种可用技术,这篇综述旨在提供适用于葡萄酒认证的最流行的光谱方法和化学计量学数据分析程序的最新信息。
    In a global context where trading of wines involves considerable economic value, the requirement to guarantee wine authenticity can never be underestimated. With the ever-increasing advancements in analytical platforms, research into spectroscopic methods is thriving as they offer a powerful tool for rapid wine authentication. In particular, spectroscopic techniques have been identified as a user-friendly and economical alternative to traditional analyses involving more complex instrumentation that may not readily be deployable in an industry setting. Chemometrics plays an indispensable role in the interpretation and modelling of spectral data and is frequently used in conjunction with spectroscopy for sample classification. Considering the variety of available techniques under the banner of spectroscopy, this review aims to provide an update on the most popular spectroscopic approaches and chemometric data analysis procedures that are applicable to wine authentication.
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  • 文章类型: Journal Article
    骨关节炎(OA)是一种退行性疾病,目前还没有有效的药物来治愈它。早期预防和治疗可有效减轻OA患者的痛苦,节约费用。因此,早期诊断OA是必要的。OA有多种诊断方法,但应用于早期诊断的方法有限。普通的光学诊断局限于表面,而实验室测试,如类风湿因子检查和物理关节炎检查,太琐碎或耗时。显然,迫切需要开发一种快速无损检测方法,用于OA的早期诊断。振动光谱学是一种快速、无损的技术,备受关注。在这次审查中,近红外(NIR),红外线,介绍了(IR)和拉曼光谱,以显示它们在早期OA诊断中的潜力。首先讨论了基本原理,然后讨论了迄今为止的研究进展,以及其局限性和发展方向。最后,对所有方法进行了比较,和振动光谱学证明它可以用作早期OA诊断的有希望的工具。本综述为振动光谱技术在OA诊断中的应用和发展提供了理论支持,为关节炎的无损快速诊断提供了新的策略,促进了基于组分的分子谱检测技术的发展和临床应用。
    Osteoarthritis (OA) is a degenerative disease, and there is currently no effective medicine to cure it. Early prevention and treatment can effectively reduce the pain of OA patients and save costs. Therefore, it is necessary to diagnose OA at an early stage. There are various diagnostic methods for OA, but the methods applied to early diagnosis are limited. Ordinary optical diagnosis is confined to the surface, while laboratory tests, such as rheumatoid factor inspection and physical arthritis checks, are too trivial or time-consuming. Evidently, there is an urgent need to develop a rapid nondestructive detection method for the early diagnosis of OA. Vibrational spectroscopy is a rapid and nondestructive technique that has attracted much attention. In this review, near-infrared (NIR), infrared, (IR) and Raman spectroscopy were introduced to show their potential in early OA diagnosis. The basic principles were discussed first, and then the research progress to date was discussed, as well as its limitations and the direction of development. Finally, all methods were compared, and vibrational spectroscopy was demonstrated that it could be used as a promising tool for early OA diagnosis. This review provides theoretical support for the application and development of vibrational spectroscopy technology in OA diagnosis, providing a new strategy for the nondestructive and rapid diagnosis of arthritis and promoting the development and clinical application of a component-based molecular spectrum detection technology.
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  • 文章类型: Journal Article
    病毒的扩散和传播已成为全球生物安全的威胁,以当前的COVID-19大流行为例。病毒感染的早期诊断和疾病控制一直至关重要。病毒检测可以基于各种等离子体现象来实现,包括传播表面等离子体共振(SPR),局部SPR,表面增强拉曼散射,表面增强荧光和表面增强红外吸收光谱。本综述涵盖了基于等离子体的病毒检测的所有可用信息,并根据几个参数收集这些传感器的数据。这些数据将帮助观众推进新一代多功能病毒生物传感器的研究和开发。
    The proliferation and transmission of viruses has become a threat to worldwide biosecurity, as exemplified by the current COVID-19 pandemic. Early diagnosis of viral infection and disease control have always been critical. Virus detection can be achieved based on various plasmonic phenomena, including propagating surface plasmon resonance (SPR), localized SPR, surface-enhanced Raman scattering, surface-enhanced fluorescence and surface-enhanced infrared absorption spectroscopy. The present review covers all available information on plasmonic-based virus detection, and collected data on these sensors based on several parameters. These data will assist the audience in advancing research and development of a new generation of versatile virus biosensors.
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