本章介绍了使用DNA序列数据获取和比较使用公共数据库GenBank和BarcodeofLifeDataSystem（BOLD）进行分类鉴定的程序。本章首先描述了用于准备上传到GenBank和BOLD的质量序列的程序。接下来,使用GenBankBLAST和BOLD识别引擎描述了用于针对公共数据库查询DNA序列的步骤。提出了分类识别分配的解释指南。最后,提供了用于评估来自GenBank和BOLD的序列的准确性和可靠性的程序。
This chapter describes procedures for the use of DNA sequence data to obtain and compare taxonomic identification using the public databases GenBank and Barcode of Life Data System (BOLD). The chapter begins by describing procedures used to prepare quality sequences for uploading into GenBank and BOLD. Next, steps used to query the DNA sequences against the public databases are described using GenBank BLAST and BOLD identification engines. Interpretation
guidelines for the taxonomic identification assignments are presented. Finally, a procedure for evaluating the accuracy and reliability of sequences from GenBank and BOLD is provided.
