微流体技术已成为各种应用中的有前途的工具,包括生物传感,疾病诊断,和环境监测。微流体装置的一个显著特征是它们能够选择性地捕获和释放特定的细胞。生物分子,细菌,和粒子。与传统的批量分析仪器相比,微流体捕获和释放平台提供了几个优点,例如非接触式操作,无标签检测,精度高,灵敏度好,和最低的试剂要求。然而,尽管过去致力于开发创新的捕获机制,捕获颗粒的释放和回收效率经常被忽视。许多先前的研究主要集中在粒子捕获技术及其效率,忽略了成功释放颗粒对后续分析的关键作用。在现实中,有效释放被捕获粒子的能力对于确保持续的,高通量分析。为了解决这个差距,这篇综述旨在强调捕获和释放机制在微流体系统中的重要性,并评估其有效性。这些方法分为两类:基于物理原理的方法和使用生化方法的方法。此外,这篇综述提供了专门设计用于颗粒捕获和释放的微流体平台的最新应用的全面总结。它概述了这些设备的设计和性能,强调它们在各种目标应用和目的中的优势和局限性。最后,审查最后讨论了该领域当前面临的挑战,并提出了潜在的未来方向。
Microfluidic technology has emerged as a promising tool in various applications, including biosensing, disease diagnosis, and environmental monitoring. One of the notable features of microfluidic devices is their ability to selectively capture and release specific cells, biomolecules, bacteria, and particles. Compared to traditional bulk analysis instruments, microfluidic capture-and-release platforms offer several advantages, such as contactless operation, label-free detection, high accuracy, good sensitivity, and minimal reagent requirements. However, despite significant efforts dedicated to developing innovative capture mechanisms in the past, the release and recovery efficiency of trapped particles have often been overlooked. Many previous studies have focused primarily on particle capture techniques and their efficiency, disregarding the crucial role of successful particle release for subsequent analysis. In reality, the ability to effectively release trapped particles is particularly essential to ensure ongoing, high-throughput analysis. To address this gap, this
review aims to highlight the importance of both capture and release mechanisms in microfluidic systems and assess their effectiveness. The methods are classified into two categories: those based on physical principles and those using biochemical approaches. Furthermore, the
review offers a comprehensive summary of recent applications of microfluidic platforms specifically designed for particle capture and release. It outlines the designs and performance of these devices, highlighting their advantages and limitations in various target applications and purposes. Finally, the
review concludes with discussions on the current challenges faced in the field and presents potential future directions.