心血管疾病(CVDs)是全球头号死因,每年夺走1790万人的生命。快速,敏感,心脏生物标志物的准确测定对心血管疾病的及时诊断至关重要。为了准确诊断,对单一生物标志物的依赖是不可靠的,因为每一种生物标志物也与其他疾病有关.为了克服这个问题,两个或两个以上标记的多重测定已成为单标记分析的有希望的替代方法.在过去的5年里,对开发靶向多种心脏标志物的生物传感器的研究兴趣已经增加。在这项研究中,我们批判性地回顾了过去5年报道的各种多路生物传感方法,通过信号读数对它们进行分类。预期检测配置,捕获探针,电极设计策略,电极类型,纳米材料,记者标签,并回顾了检测类型,列表,并进行了批判性讨论。然后,强调了它们的优点和局限性。对于每个类别,我们提供了我们的观点以及整体的批判性讨论。最后,我们总结了潜在的商业多路复用心脏生物传感器,并评论了此类传感器的挑战和未来前景。
Cardiovascular diseases (CVDs) are the number one cause of death worldwide, taking 17.9 million lives each year. The rapid, sensitive, and accurate determination of cardiac biomarkers is vital for the timely diagnosis of CVDs. For accurate diagnosis, dependence on a single biomarker is unreliable because each one has also been linked to other diseases. To overcome this problem, the multiplexed determination of two or more markers has emerged as a promising alternative to single-marker analysis. Over the last 5 years, research interest in the development of biosensors for targeting multiple cardiac markers has increased. In this study, we critically reviewed the various multiplexed biosensing approaches reported during the last 5 years, categorizing them by signal readouts. Prospective detection configurations, capture probes, electrode design strategies, electrode types, nanomaterials, reporter tags, and assay types were reviewed, tabulated, and critically discussed. Then, their advantages and limitations were highlighted. For each category, we provided our perspective as well as the overall critical discussion. Lastly, we summarized potential commercial multiplexed cardiac biosensors and commented on the challenges and future prospects for such sensors.