■本研究的目的是通过网络药理学研究人参五味子汤加减治疗反复呼吸道感染(RRTI)的作用机制。
■对一种改性人参五味子汤的有效成分进行筛选,TCMSP,TCMID,应用Batman-TCM和PubChem数据库。为了预测活性成分对RRTI的影响,TCMSP,Pubmed,OMIM,毒品银行,使用GAD和TTD数据库。用Cytoscape3.7.2软件构建化合物-治疗靶点网络。STRING数据库用于构建蛋白质-蛋白质相互作用(PPI)网络,和京都基因和基因组百科全书(KEGG)途径富集分析用于鉴定潜在的信号途径。
■经筛选得到的改性人参五味子汤的3种主要活性成分为槲皮素,山奈酚,和异黄酮;主要治疗靶点是PTGS2,ESR1,AR,PPARG,NOS2和其他。基于PPI网络,我们发现加味人参五味子汤的作用靶点显著丰富(FDR<0.01),肿瘤坏死因子(TNF)信号通路,缺氧诱导因子(HIF-1)信号通路,和其他人。
■加味人参五味子汤主要通过作用于肿瘤通路和TNF通路的一些关键节点的信号转导来治疗RRTI。它对多种信号通路产生直接或间接的影响,具有多组分的特点,多目标,和多通道行动。
UNASSIGNED: The aim of this
study was to investigate the mechanism of Modified Ginseng-Schisandra Decoction in the treatment of recurrent respiratory tract infection (RRTI) using network pharmacology.
UNASSIGNED: To screen the active ingredients of A Modified Ginseng-Schisandra Decoction, TCMSP, TCMID, Batman-TCM and PubChem database were applied. To predict the targets of active ingredients on RRTI, TCMSP, Pubmed, OMIM, Drug Bank, GAD and TTD database were used. The compounds-therapeutic target network was constructed with Cytoscape 3.7.2 software. The STRING database was used to construct a protein-protein interaction (PPI) network, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was used to identify potential signal pathways.
UNASSIGNED: The 3 main active ingredients of Modified Ginseng-Schisandra Decoction obtained by screening were quercetin, kaempferol, and isoflavone; the main therapeutic targets were PTGS2, ESR1, AR, PPARG, NOS2, and others. Based on the PPI network, we found that the targets of Modified Ginseng-Schisandra Decoction were significantly enriched in (FDR <0.01) cancer pathway, tumor necrosis factor (TNF) signaling pathway, hypoxia-inducible factor (HIF-1) signaling pathway, and others.
UNASSIGNED: Modified Ginseng-Schisandra Decoction can treat RRTI primarily through acting in the signal transduction of some key nodes of cancer pathway and TNF pathway. It exerts a direct or indirect influence on multiple signaling pathways, and has the characteristics of multicomponent, multitarget, and multichannel action.