2019年冠状病毒病(COVID-19)是一种严重的疾病,由首次出现在中国的β冠状病毒的一种新变种引起。人类的遗传因素,包括多态性,在SARS-CoV-2的高传播和在一小部分但很大一部分感染者中看到的顽固进展的疾病中起着关键作用;然而,但是这些因素仍然不明确。使用限制性片段长度多态性聚合酶链反应(RFLP-PCR)和扩增难治性突变系统(ARMS)-PCR技术,对288例COVID-19患者和288例对照进行了TMPRSS2多态性的基因分型。在疾病易感性和死亡率方面比较了不同基因型的TMPRSS2多态性。统计分析表明,除rs75603675C>A变体外,所有研究变体的次要等位基因在统计学上增加了COVID-19的风险。rs12329760的T等位基因增加了COVID-19的风险。此外,基因型的AG/AC/TT/AG组合显着增加了我们人群中COVID-19的风险。不同单倍型rs17854725/rs75603675/rs12329760/rs4303795多态性,包括GACA,GACG,GATG,GATA,AATA,ACCG,ACTG,ACTA,GCCA,和GCTG,被发现与疾病风险增加相关(比值比>1)。关于临床和临床特征,除性别外,非严重和严重形式之间存在统计学上的显着差异,血小板,C反应蛋白(CRP),红细胞沉降率(ESR),和潜在的疾病。此外,TMPRSS2rs17854725A>G的病例基因型,rs12329760C>T,rs4303795A>G在严重和非严重疾病方面存在显着差异(P值<0.001)。具体来说,在rs17854725A>G的AG基因型携带者中死亡更为频繁(P值=0.022)。携带所研究的四种TMPRSS2变体的次要等位基因的患者相当容易感染COVID-19。我们的发现表明rs17854725A>G(AA与AG和AAvs.GG),rs12329760C>T(CCvs.CT和CCvs.TT),rs4303795A>G(AAvs.AG)TMPRSS2变异的基因型与更具侵袭性的疾病模式有关。需要对更大的人群进行更多的研究来证实我们的结果。
Coronavirus disease 2019 (COVID-19) is a severe disease caused by a new variant of beta-coronavirus that first appeared in China. Human genetic factors, including polymorphisms, serve pivotal roles in the high transmission of SARS-CoV-2 and the stubbornly progressing sickness seen in a small but significant percentage of infected people; however, but these factors remain ill-defined. A total of 288 COVID-19 patients and 288 controls were genotyped for TMPRSS2 polymorphisms using both restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) and amplification refractory mutation system (ARMS)-PCR techniques. Different genotypes of TMPRSS2 polymorphisms were compared in terms of disease susceptibility and mortality. The statistical analysis showed that minor alleles of all studied variants statistically increased the risk of COVID-19, except for the rs75603675 C > A variant. The T allele of rs12329760 conferred an increased risk of COVID-19. Moreover, the AG/AC/TT/AG combination of genotypes significantly enhanced the risk of COVID-19 in our population. Different haplotypes of rs17854725/rs75603675/rs12329760/rs4303795 polymorphisms, including GACA, GACG, GATG, GATA, AATA, ACCG, ACTG, ACTA, GCCA, and GCTG, were found to be associated with increased risk of the disease (odds ratio > 1). Regarding the clinical and paraclinical characteristics, a statistically significant difference was found between non-severe and severe forms except for gender, platelet, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and underlying diseases. In addition,
case genotypes of TMPRSS2 rs17854725 A > G, rs12329760 C > T, and rs4303795 A > G were significantly different regarding severe and non-severe forms of the disease (P-value < 0.001). Specifically, death was more frequent in carriers of the AG genotype of rs17854725 A > G (P-value = 0.022). Patients who carry the minor alleles of the four studied TMPRSS2 variants were rather vulnerable to COVID-19 infection. Our findings indicated that rs17854725 A > G (AA vs. AG and AA vs. GG), rs12329760 C > T (CC vs. CT and CC vs. TT), and rs4303795 A > G (AA vs. AG) genotypes of TMPRSS2 variations are associated with a more invasive disorder pattern. More studies on larger populations are needed to confirm our results.