麻醉是减轻水产养殖和产品运输过程中水生动物应激反应的有效方法。在这项研究中,我们评估了丁香油的麻醉效果,甲磺酸三卡因(MS-222),乙醇,和氯化镁通过麻醉持续时间,恢复时间,24小时生存率,和泥蟹(Scyllaparamamosain)的行为。此外,还研究了不同体重泥蟹的最佳麻醉浓度。结果表明,丁香油是泥蟹的最佳麻醉剂,24小时存活率超过MS-222和氯化镁治疗中观察到的存活率。乙醇导致泥蟹截肢和多动症。丁香油的最佳麻醉浓度与泥蟹的0.03-27.50g和27.50-399.73g重量类别之间的回归分析得出以下方程式:y=0.0036x3-0.1629x21.7314x4.085(R2=0.7115)和y=0.0437x2.9461(R2=0.9549)。丁香油对血清皮质醇无显著影响,葡萄糖,乳酸含量,天冬氨酸转氨酶(AST),丙氨酸氨基转移酶(ALT)活性,或超氧化物歧化酶(SOD),过氧化氢酶(CAT),谷胱甘肽过氧化物酶(GSH-Px),不同处理组泥蟹中丙二醛(MDA)水平。泥蟹中丁香油诱导的麻醉导致抑制性神经递质如甘氨酸的增加。然而,麻醉恢复与兴奋性神经递质L-天冬氨酸和谷氨酸水平升高相关.总之,丁香油被证明是泥蟹的安全和最佳麻醉剂,对物种不施加生理压力。
Anesthesia serves as an effective method to mitigate the stress response in aquatic animals during aquaculture and product transportation. In this
study, we assessed the anesthetic efficacy of clove oil, tricaine methane-sulfonate (MS-222), ethanol, and magnesium chloride by anesthesia duration, recovery time, 24-hour survival rate, and the behavior of mud crabs (Scylla paramamosain). Additionally, the optimal anesthetic concentration for varying body weights of mud crabs was also investigated. The results revealed that clove oil emerged as the optimal anesthetic for mud crabs, with a 24-hour survival rate surpassing those observed in MS-222 and magnesium chloride treatments. Ethanol caused amputation and hyperactivity in mud crabs. Regression analyses between the optimal anesthetic concentration of clove oil and the weight categories of 0.03-27.50 g and 27.50-399.73 g for mud crabs yielded the following equations: y = 0.0036 x3 - 0.1629 x2 + 1.7314 x + 4.085 (R2 = 0.7115) and y = 0.0437 x + 2.9461 (R2 = 0.9549). Clove oil exhibited no significant impact on serum cortisol, glucose, lactate content, aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities, or superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels in mud crabs across different treatment groups. Anesthesia induced by clove oil in mud crabs resulted in an increase in inhibitory neurotransmitters such as glycine. However, the recovery from anesthesia was associated with elevated levels of the excitatory neurotransmitters L-aspartic acid and glutamate. In conclusion, clove oil proves to be a safe and optimal anesthetic agent for mud crabs, exerting no physiological stress on the species.