基因测序,或DNA测序,使用Sanger技术已广泛用于兽医诊断界。该技术在验证PCR结果方面发挥作用,用于提供系统发育分析所需的遗传序列数据,流行病学研究,和法医调查。美国兽医实验室诊断医师协会的实验室技术委员会制定了样品制备指南,提交给测序设施或仪器,对核酸序列数据进行质量评估,并用于生成诊断应用的基本测序数据和系统发育分析。本指南旨在协助实验室提供一致,高品质,使用基于Sanger的基因测序作为实验室服务的组成部分时,以及可靠的序列数据。
Genetic sequencing, or DNA sequencing, using the Sanger technique has become widely used in the veterinary diagnostic community. This technology plays a role in verification of PCR results and is used to provide the genetic sequence data needed for phylogenetic analysis, epidemiologic studies, and forensic investigations. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians has prepared
guidelines for sample preparation, submission to sequencing facilities or instrumentation, quality assessment of nucleic acid sequence data performed, and for generating basic sequencing data and phylogenetic analysis for diagnostic applications. This guidance is aimed at assisting laboratories in providing consistent, high-quality, and reliable sequence data when using Sanger-based genetic sequencing as a component of their laboratory services.