用于筛查和诊断口腔扁平苔藓(OLP)的唾液蛋白生物标志物尚不明确。这项研究的目的是使用蛋白质组学方法鉴定OLP的推定蛋白质生物标志物。
收集来自5名OLP患者和5名健康对照参与者的未刺激的全唾液。然后对唾液样品进行二维凝胶电泳,然后通过质谱来鉴定推定的蛋白质生物标志物。随后,在24名OLP患者和24名年龄匹配的健康对照受试者中验证了这些推定的生物标志物的子集,使用酶联免疫吸附测定(ELISA)。然后在3对年龄和性别匹配的OLP患者和健康对照中进行免疫印迹分析以确认来自ELISA研究的结果。
鉴定出31个蛋白质点,对应于20种独特的蛋白质。值得注意的是,纤维蛋白原片段D和补体成分C3c在OLP患者中表达增加,虽然胱抑素SA在OLP患者中表达降低,与健康对照组相比。ELISA分析表明纤维蛋白原片段D和补体成分C3c的表达增加,胱抑素SA的表达减少,在OLP患者的唾液中。在OLP患者和健康对照受试者之间观察到唾液补体C3c表达的统计学差异。免疫印迹分析证实了我们的ELISA研究的结果。
补体C3c,纤维蛋白原片段D和胱抑素SA可作为唾液生物标志物用于筛查和/或诊断OLP.
Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches.
Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers. Subsequently, a subset of these putative biomarkers were validated in 24 OLP patients and 24 age-matched healthy control subjects, using an enzyme-linked immunosorbent assay (ELISA). Immunoblotting analyses were then performed in 3 pairs of age- and sex-matched OLP patients and healthy controls to confirm results from the ELISA study.
Thirty-one protein spots were identified, corresponding to 20 unique proteins. Notably, fibrinogen fragment D and complement component C3c exhibited increased expression in OLP patients, while cystatin SA exhibited decreased expression in OLP patients, compared with healthy control subjects. ELISA analyses indicated increased expression of fibrinogen fragment D and complement component C3c, and decreased expression of cystatin SA, in the saliva of OLP patients. Statistical differences in the expression of salivary complement C3c were observed between OLP patients and healthy control subjects. Immunoblotting analyses confirmed the results of our ELISA study.
Complement C3c, fibrinogen fragment D and cystatin SA may serve as salivary biomarkers for screening and/or diagnosis of OLP.