目的:本研究的目的是确定锰超氧化物歧化酶(Mn-SOD)基因C47T多态性与伊拉克吸烟者恶性肺癌风险之间的关系。
方法:从260名肺癌患者(88名女性和172名男性)和295名健康个体(91名女性和204名男性)获得血液样本。从血液样品中提取DNA并使用特异性引物扩增SOD2基因。通过使用国家生物技术信息中心(NCBI)的BLAST服务器和Raptorx应用程序分析SOD2基因的核苷酸序列。
结果:TT,CT,CC基因型浓度为48.1%,33.2%,和18.7%,分别,在对照组中。TT的浓度,CT,CC基因型占43.5%,31.5%,25%,分别,在案件组。在SOD2C47T多态性的基因型频率方面,病例与对照组之间没有统计学差异。我们观察到,与TT基因型相比,SOD2C47T多态性CT基因型并未增加肺癌发展的风险(OR=0.951,95%CI=0.648-1.396;P=0.798)。此外,研究发现,与TT基因型相比,CC基因型并未增加肺癌的发生风险(OR=0.673,95%Cl=0.435~1.041:P=0.075).
结论:这些结果表明,在伊拉克吸烟者中,SOD2C47T多态性与肺癌发展风险之间没有关联。
OBJECTIVE: The objective of this study was to determine the association between manganese superoxide dismutase (Mn-SOD) gene C47 T polymorphism and the risk of malignant lung cancer in Iraqi smokers.
METHODS: Blood samples were obtained from 260 lung cancer patients (88 females and 172 males) and 295 healthy individuals (91 females and 204 males). DNA was extracted from blood samples and the
SOD2 gene was amplified using specific primers. The nucleotide sequences of the
SOD2 gene were analyzed by using BLAST server at National Center for Biotechnology Information (NCBI) and the Raptorx app.
RESULTS: TT, CT, and CC genotypes concentrations were 48.1%, 33.2%, and 18.7%, respectively, in the control group. The concentrations of TT, CT, and CC genotypes were 43.5%, 31.5%, and 25%, respectively, in the
case group. There were no statistical differences between cases and controls in terms of genotype frequency of SOD2C47T polymorphism. We observed that SOD2C47T polymorphism CT genotype did not increase the risk of lung cancer development compared to those with TT genotype (OR= 0.951, 95% CI = 0.648-1.396; P = 0.798). In addition, it was observed that CC genotype did not increase the risk of lung cancer development in comparison with TT genotype ( OR=0.673, 95% Cl=0.435-1.041: P=0.075).
CONCLUSIONS: These results indicated that there was no association between SOD2C47T polymorphism and the risk of lung cancer development in Iraqi smokers.