该研究的目的是通过病例对照研究评估CD40/CD40配体(CD40L)和CD134/CD134配体(CD134L)在冠心病(CHD)发展中的潜在作用。研究对象为234例CHD患者和120例匹配良好的正常对照者。外周血单核细胞(PBMC)分离后,实时定量PCR(qRT-PCR),蛋白质印迹,免疫组织化学,和流式细胞术检测CD40/CD40L和CD134/CD134L的mRNA水平和表达水平;同时,qRT-PCR检测细胞间粘附分子-1(ICAM-1)和Fas蛋白mRNA水平。组间基线特征比较无统计学差异,表明群体之间的可比性。qRT-PCR和Westernblot分析表明,CHD组CD40/CD40L和CD134/CD134LmRNA和蛋白表达水平均高于对照组。流式细胞术进一步证实了类似的趋势。同时,冠心病组ICAM-1和Fas蛋白mRNA水平升高,且与上述指标呈正相关。此外,CD40/CD40L表达率与性别、不同类型CHD呈负相关。同时,CD134/CD134L在男性患者中的表达也较高,在有家族史的患者中,既往高血压史,糖尿病,和脑血管疾病。CD40/CD40L和CD134/CD134L升高,可能与冠心病患者的临床病理特征有关。需要结合体内和体外实验进一步深入探索用于CHD指导的共刺激分子以及内在机制。
The aim of the study was to evaluate the potential role of CD40/CD40 ligand (CD40L) and CD134/CD134 ligand (CD134L) in the development of coronary heart disease (CHD) via the performance of a
case-control study.The research objects were 234 cases of CHD patients and 120 cases of well-matched normal controls. Following the separation of peripheral blood mononuclear cells (PBMCs), real-time quantitative PCR (qRT-PCR), Western blot, immunohistochemistry, and flow cytometry were applied for the detection of mRNA levels and expression levels of CD40/CD40L and CD134/CD134L; meanwhile, intercellular adhesion molecule-1 (ICAM-1) and Fas protein mRNA levels were detected using qRT-PCR.There was no statistical difference in the comparison of baseline characteristics between groups, indicating comparability between groups. qRT-PCR and Western blot analysis indicated that CD40/CD40L and CD134/CD134L mRNA and protein expression levels were all increased in the CHD group than those in the control group. Flow cytometry further confirmed the similar tendency. Meanwhile, ICAM-1 and Fas protein mRNA levels were elevated in the CHD group and positively correlated with the above parameters. Furthermore, CD40/CD40L expression rates were negatively correlated with gender and different types of CHD. Meanwhile, CD134/CD134L expressions were also higher in male patients, in patients with family history, previous history of hypertension, diabetes, and cerebrovascular diseases.CD40/CD40L and CD134/CD134L are increased and may have potential correlation with clinical pathological features of patients with CHD. Further in-depth exploration of costimulatory molecules for CHD guidance as well as intrinsic mechanisms are needed combined with in vivo and in vitro experiments.