Duvelisib(DUV)是一种有效的抗癌药物,而莫西沙星(MOX)是一种具有抗癌细胞增殖能力的抗菌药物,这是在癌症治疗中根据经验进行的。DUV和MOX组合通常用于对抗患者在化疗期间的感染。这项研究描述了,第一次,开发了一种简单而绿色的同步荧光光谱(SSF)方法,用于同时估计血浆中的DUV和MOX。DUV和MOX在273和362nm处定量,在Δλ为120nm时彼此无干扰。深入研究了影响荧光强度的实验变量,并建立了最佳条件。在pH3.5时,在水溶剂中使用乙酸钠缓冲溶液获得最佳同步荧光强度(SFI)。DUV和MOX的校准曲线,将SFI与相应的药物浓度相关联,两种药物在50-1000ngmL-1范围内呈线性关系,具有良好的相关系数。该方法非常灵敏,DUV和MOX的检出限为24和22ngmL-1,定量限为40和45ngmL-1,分别。SSF方法根据食品和药物管理局(FDA)的分析程序验证指南进行验证,和验证参数是可接受的。拟议的SSF方法用于单次同时口服两种药物后大鼠血浆的药代动力学和生物利用度研究。研究结果表明,与MOX同时施用时,应谨慎使用DUV剂量。SSF方法的绿色性通过三种不同的度量工具进行评估,即分析生态尺度,绿色分析程序索引,和分析绿色计算器。结果表明,SSF方法是一种生态友好的绿色分析方法。总之,所提出的SSF方法是同时给药的DUV和MOX的药代动力学/生物利用度研究和治疗药物监测的有价值的工具。
.
Duvelisib (DUV) is a potent anticancer drug whereas Moxifloxacin (MOX) is an antimicrobial drug with anti-proliferative potency against cancerous cells, which is empirically administered in cancer treatment. DUV and MOX combination is commonly prescribed to combat infections in patients while they are under chemotherapy treatment. This
study describes, for the first time, the development of a simple and green synchronous spectrofluorimetric (SSF) method for the simultaneous estimation of DUV and MOX in plasma. DUV and MOX were quantified at 273 and 362 nm, respectively without interference between each other at Δλof 120 nm. The experimental variables influencing fluorescence intensities were thoroughly investigated and the optimum conditions were established. At pH 3.5, the optimum synchronous fluorescence intensity (SFI) was achieved in water solvent by using sodium acetate buffer solution. Calibration curves for DUV and MOX, correlating the SFI with the corresponding drug concentration, were linear in the range of 50-1000 ng mL-1for both drugs, with good correlation coefficients. The method was extremely sensitive, with limits of detection of 24 and 22 ng mL-1, and limits of quantitation of 40 and 45 ngmL-1for DUV and MOX, respectively. The SSF method was validated according to the Food and Drug Administration (FDA) guidelines for validation of analytical procedures, and the validation parameters were acceptable. The proposed SSF method was applied to the pharmacokinetic and bioavailability studies in rats\' plasma after single concurrent oral administration of both drugs. The results of the
study revealed that caution should be taken with DUV dose when concurrently administered with MOX. The greenness of SSF method was assessed by three different metric tools namely Analytical Eco-scale, Green Analytical Procedure Index, and Analytical Greenness Calculator. The results confirmed that SSF method is an eco-friendly and green analytical approach. In conclusion, the proposed SSF method is a valuable tool for pharmacokinetic/bioavailability studies and therapeutic drug monitoring of simultaneously administered DUV and MOX.