光学清除剂(OCA)是暂时改变组织的光学性质的物质,使更好的成像和光穿透。本研究旨在使用体内和体外光学方法评估OCA对甲床和血液的影响。在体内部分,将OCA涂在甲床上,光学相干层析成像和光学数字毛细血管镜检查用于评估其对光学清除和毛细血管血流的影响,分别。在体外部分,将收集的血液样品与OCA孵育,并使用漫射光散射技术评估血液聚集特性.结果表明,OCA显着影响甲床的光学性能和血液微流变学。这些发现表明,OCA有望改善光学成像和诊断,特别是对于甲床应用,并能改变血液微流变学。
Optical clearing agents (OCAs) are substances that temporarily modify tissue\'s optical properties, enabling better imaging and light penetration. This
study aimed to assess the impact of OCAs on the nail bed and blood using in vivo and in vitro optical methods. In the in vivo part, OCAs were applied to the nail bed, and optical coherence tomography and optical digital capillaroscopy were used to evaluate their effects on optical clearing and capillary blood flow, respectively. In the in vitro part, the collected blood samples were incubated with the OCA and blood aggregation properties were estimated using diffuse light scattering techniques. The results indicate that OCAs significantly influence the optical properties of the nail bed and blood microrheology. These findings suggest that OCAs hold promise for improving optical imaging and diagnostics, particularly for nail bed applications, and can modify blood microrheology.