Quantitative methods

定量方法
  • 文章类型: Journal Article
    花青素,黄酮类化合物的一个子类,以其鲜艳的颜色和促进健康的特性而闻名,在营养科学和食品工业中至关重要。这篇综述文章深入研究了食品基质中花青素检测和定量的分析方法,比较定量和局部技术。定量方法,包括高效液相色谱(HPLC)和质谱(MS),提供单个花青素的精确定量和分析,但需要样品破坏,限制其在连续质量控制中的使用。局部方法,如近红外光谱(NIR)和高光谱成像,提供快速,在不损害样品完整性的情况下进行原位分析,理想的现场食品质量评估。这篇综述强调了色谱技术的进步,特别是超高效液相色谱(UHPLC)与现代检测器,提高花色苷分析的分辨率和速度。它还强调了局部技术在食品工业中的效率和最少的样品制备的重要性。通过检查两个分析领域的优势和局限性,本文旨在阐明当前的挑战和未来的进步,为改善食品中花青素的分析提供未来研究方向的见解。
    Anthocyanins, a subclass of flavonoids known for their vibrant colors and health-promoting properties, are pivotal in the nutritional science and food industry. This review article delves into the analytical methodologies for anthocyanin detection and quantification in food matrices, comparing quantitative and topical techniques. Quantitative methods, including High-performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), offer precise quantification and profiling of individual anthocyanins but require sample destruction, limiting their use in continuous quality control. Topical approaches, such as Near-infrared Spectroscopy (NIR) and hyperspectral imaging, provide rapid, in situ analysis without compromising sample integrity, ideal for on-site food quality assessment. The review highlights the advancements in chromatographic techniques, particularly Ultra-high-performance Liquid Chromatography (UHPLC) coupled with modern detectors, enhancing resolution and speed in anthocyanin analysis. It also emphasizes the growing importance of topical techniques in the food industry for their efficiency and minimal sample preparation. By examining the strengths and limitations of both analytical realms, this article aims to shed light on current challenges and prospective advancements, providing insights into future research directions for improving anthocyanin analysis in foods.
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  • 文章类型: Systematic Review
    目的:当前的召回期选择指南建议考虑研究的设计,条件的性质,患者的负担和回忆能力,和结果度量的意图。建议对召回期的准确性进行实证研究;然而,对于如何定量评估不同召回期患者报告结局指标(PROMs)结果的一致性,目前尚无共识.我们进行了系统的审查,以描述评估从不同召回期的PROM获得的结果的定量方法,为建立共识奠定基础。
    方法:我们搜索了MEDLINE,Embase,Scopus,和APAPsycINFO用于研究参与者被给予相同的健康相关措施(例如,生活质量,幸福,功能,和痛苦)有不同的召回期。
    结果:共筛选了7,174篇摘要。纳入的30项研究反映了广泛的领域,包括疼痛,疲劳,性行为和性功能。召回期从短暂到六个月不等。分析方法各不相同,包括评估相对协议的不同方法,绝对协议,并用于评估相对和绝对一致性。
    结论:我们发现评估PROM召回期的方式存在差异,建议有机会就方法论方法达成更大的共识。作为起点,我们提供了哪些方法更适合哪些环境的建议。
    The current guidance for selection of recall periods recommends considering the design of the study, nature of the condition, patient\'s burden and ability to recall, and intent of the outcome measure. Empirical study of the accuracy of recall periods is recommended; however, there is not consensus on how to quantitatively evaluate the consistency of results from patient-reported outcome measures (PROMs) with different recall periods. We conducted a systematic review to describe quantitative methods for evaluating results obtained from PROMs with differing recall periods to lay the groundwork for establishing consensus.
    We searched MEDLINE, Embase, Scopus, and American Psychological Association PsycINFO for studies where participants are given the same health-related measure (eg, quality of life, well-being, functioning, and pain) with differing recall periods.
    A total of 7174 abstracts were screened. The 30 included studies reflected a wide range of domains, including pain, fatigue, and sexual behavior and function. The recall periods ranged from momentary to 6 months. The analytic approaches varied, including different methods for assessing relative agreement, absolute agreement, and for assessing combined relative and absolute agreement.
    We found variability in how PROM recall periods were evaluated, suggesting an opportunity for greater consensus on methodological approach. As a starting point, we provide recommendations for which methods are preferred for which contexts.
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  • 文章类型: Journal Article
    快速的气候和社会经济变化正在改变北极人类-地球系统。这些系统的一个组成部分是移动性,其中包括人类和货物的运输,出,和北极地区之间。气候和社会经济驱动因素对北极交通的影响是异质的。需要采取各种方法,在可以与更广泛的社会经济体系相联系的措施中量化这些影响。本文回顾了现有的方法,并将它们组织成一个概念框架,以了解文献中的趋势和差距。我们找到了量化一系列气候驱动因素对北极大多数交通方式影响的方法,但很少有方法关注社会经济驱动因素。此外,代表性不足的是明确考虑运输系统自适应能力的方法。我们提供与理解北极变化对运输系统影响相关的数据和关系的洞察,为研究这些影响如何适应更广泛的人类系统的未来工作奠定基础。
    Rapid climate and socioeconomic changes are transforming Arctic human-earth systems. An integral part of these systems is mobility, which encompasses the transport of humans and goods into, out of, and between Arctic regions. Impacts of climate and socioeconomic drivers on Arctic mobility are heterogenous. Methodologies are needed to quantify these impacts in measures that can be linked with broader socioeconomic systems. This article reviews existing methods and organizes them into a conceptual framework to understand trends and gaps in the literature. We found methods quantifying impacts of a range of climate drivers on most transportation modes present in the Arctic, but few methods focused on socioeconomic drivers. In addition, underrepresented were methods explicitly considering adaptive capacity of transportation systems. We provide insight into the data and relationships relevant to understanding impacts of Arctic change on transportation systems, laying a foundation for future work that investigates how these impacts fit into broader human-arth systems.
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  • 文章类型: Editorial
    《病理学杂志》2022年年度评论,病理学的最新进展,包含15篇关于病理学中日益重要的研究领域的特邀评论。今年,这些文章包括那些专注于数字病理学的文章,采用现代成像技术和软件来改进诊断和研究应用,以研究人类疾病。该主题领域包括通过其诱导的形态变化来识别特定遗传改变的能力,以及将数字和计算病理学与组学技术集成。本期的其他评论包括对癌症突变模式(突变特征)的最新评估,谱系追踪在人体组织中的应用,和单细胞测序技术来揭示肿瘤进化和肿瘤异质性。组织微环境包含在专门处理表皮分化的蛋白水解控制的综述中,癌症相关成纤维细胞,场抵消,和决定肿瘤免疫的宿主因子。本期中包含的所有评论都是受邀专家的工作,这些专家被选中讨论各自领域的最新进展,并且可以在线免费获得(https://onlinelibrary。wiley.com/journal/10969896)。©2022英国和爱尔兰病理学会。由JohnWiley&Sons出版,Ltd.
    The 2022 Annual Review Issue of The Journal of Pathology, Recent Advances in Pathology, contains 15 invited reviews on research areas of growing importance in pathology. This year, the articles include those that focus on digital pathology, employing modern imaging techniques and software to enable improved diagnostic and research applications to study human diseases. This subject area includes the ability to identify specific genetic alterations through the morphological changes they induce, as well as integrating digital and computational pathology with \'omics technologies. Other reviews in this issue include an updated evaluation of mutational patterns (mutation signatures) in cancer, the applications of lineage tracing in human tissues, and single cell sequencing technologies to uncover tumour evolution and tumour heterogeneity. The tissue microenvironment is covered in reviews specifically dealing with proteolytic control of epidermal differentiation, cancer-associated fibroblasts, field cancerisation, and host factors that determine tumour immunity. All of the reviews contained in this issue are the work of invited experts selected to discuss the considerable recent progress in their respective fields and are freely available online (https://onlinelibrary.wiley.com/journal/10969896). © 2022 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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  • 文章类型: Journal Article
    In light of the many robust quantitative data sets that include information on attitudes and behaviors related to intimate partner violence (IPV), and in an effort to expand the evidence base around social norms and IPV, many researchers construct proxy measures of norms within and across groups embedded in the data. While this strategy has become increasingly popular, there is no standardized approach for assessing and constructing these norm proxies, and no review of these approaches has been undertaken to date. This study presents the results of a systematic review of methods used to construct quantitative proxy measures for social norms related to IPV. PubMed, Embase, Popline, and Scopus, and PsycINFO were searched using Boolean search techniques. Inclusion criteria comprised studies published since 2000 in English that either (i) examined a norm proxy related to gender or IPV or (ii) analyzed the relationship between a norm proxy and perpetration of, experiences of, or attitudes toward IPV. Studies that employed qualitative methods or that elicited direct measures of descriptive or injunctive norms were not included. Twenty-six studies were eligible for review. Evidence from this review highlights inconsistencies in how proxies are constructed, how they are assessed to ensure valid representation of norms, and how researchers acknowledge their respective method\'s limitations. Key processes and reflections employed by some of the studies are identified and recommended for future research inquiries.
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  • 文章类型: Journal Article
    Quantitative health disparities research has increasingly employed intersectionality as a theoretical tool to investigate how social characteristics intersect to generate health inequality. Yet, intersectionality was not designed to quantify, predict, or identify health disparities, and, as a result, multiple criticisms against its misapplication in health disparities research have been made. As such, there is an emerging need to evaluate the growing body of quantitative research that aims to investigate health disparities through an intersectional lens.
    We conducted a systematic review from earliest records to January 2020 to (i) describe the scope of limitations when applying intersectionality to quantitative health disparities research, and (ii) identify recommendations to improve the future integration of intersectionality with this scholarship. We identified relevant publications with electronic searches in PubMed and CA Web of Science. Studies eligible for inclusion were English-language publications that used quantitative methodologies to investigate health disparities among adults in the U.S. while explicitly claiming to adopt an intersectional perspective. Out of 1279 articles reviewed, 65 were eligible for inclusion.
    Our review found that, while the value of intersectionality to the study of health disparities is evident, the existing research struggles with meeting intersectionality\'s fundamental assumptions. In particular, four limitations were found to be widespread: narrowing the measurements of intersectionality, intersectional groups, and health outcomes; placing primacy on the study of certain intersectional groups to the neglect of others; overlooking underlying explanatory mechanisms that contribute to the health disparities experienced by intersectional groups; and, lacking in the use of life-course perspectives to show how health disparities vary across different life stages.
    If the goal of health equality is to be achieved among diverse intersectional groups, future research must be assisted by the collection and examination of data that overcomes these limitations.
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  • 文章类型: Journal Article
    Research in the field of health promotion generates evidence-based knowledge and has the potential to increase the acceptance, impact and long-term effectiveness of interventions. Typically, a distinction is made between qualitative and quantitative approaches. Yet, with complex and multidimensional re-search questions in the field of health promotion a combination of the two approaches has proven useful. Using mixed-methods designs promises, among other things, to compensate for weaknesses of the individual methods, to improve the scope of the results, and to provide a comprehensive insight into the topic. In this systematic review of two German journals we examine the role of the mixed-methods approach in the field of health promotion and how it can be translated into specific research projects. The review\'s results show that mixed-methods designs are implemented in various contexts. In most studies, the quantitative research strand plays the central role. The use of the mixed-methods designs in health promotion can be further discussed, especially taking into account technical innovations in the field of data collection or with regard to the interpretative potential of qualitative research. The presentation of the review is based on PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) by the Equator Network.
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  • 文章类型: Journal Article
    Target audience ratings of the likely impact of persuasive messages, known as perceived message effectiveness (PME), are commonly used during message development and selection. PME is also used to examine receptivity of messages after they are fully developed or deployed. Despite this, we know little about the conceptual and methodological characteristics of extant PME measures used in the literature. We conducted a systematic review of tobacco education video, print, and audio campaign studies to examine conceptual and methodological characteristics of PME measures. One hundred twenty-six PME measures from 75 studies conducted in 21 countries with more than 61,000 participants were reviewed. Results indicated considerable variability in measures\' focus on general perceptions of a message (i.e., message perceptions) versus perceptions of expected message effects (i.e., effects perceptions). Considerable variability was also found on underlying persuasive constructs, use of referents, and referencing of behavior in PME items and measures. We conclude with several recommendations for future research on PME measurement and validation.
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  • 文章类型: Journal Article
    The rapid expansion of research on Brain-Computer Interfaces (BCIs) is not only due to the promising solutions offered for persons with physical impairments. There is also a heightened need for understanding BCIs due to the challenges regarding ethics presented by new technology, especially in its impact on the relationship between man and machine. Here we endeavor to present a scoping review of current studies in the field to gain insight into the complexity of BCI use. By examining studies related to BCIs that employ social research methods, we seek to demonstrate the multitude of approaches and concerns from various angles in considering the social and human impact of BCI technology.
    For this scoping review of research on BCIs\' social and ethical implications, we systematically analyzed six databases, encompassing the fields of medicine, psychology, and the social sciences, in order to identify empirical studies on BCIs. The search yielded 73 publications that employ quantitative, qualitative, or mixed methods.
    Of the 73 publications, 71 studies address the user perspective. Some studies extend to consideration of other BCI stakeholders such as medical technology experts, caregivers, or health care professionals. The majority of the studies employ quantitative methods. Recurring themes across the studies examined were general user opinion towards BCI, central technical or social issues reported, requests/demands made by users of the technology, the potential/future of BCIs, and ethical aspects of BCIs.
    Our findings indicate that while technical aspects of BCIs such as usability or feasibility are being studied extensively, comparatively little in-depth research has been done on the self-image and self-experience of the BCI user. In general there is also a lack of focus or examination of the caregiver\'s perspective.
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  • 文章类型: Journal Article
    OBJECTIVE: To examine discrimination and resilience experiences of people who identify as transgender and establish potential health service responses.
    BACKGROUND: People who identify as transgender face many challenges in society in terms of the knowledge, understanding and acceptance of a person\'s gender identity.
    METHODS: A narrative review of quantitative empirical research.
    METHODS: A comprehensive search of CINAHL, MEDLINE, PsycINFO and Sociological Abstracts electronic databases from 2006-2016 was conducted.
    RESULTS: The search yielded 1,478 papers and following the application of rigorous inclusion and exclusion criteria a total of 19 papers were included in the review. The findings reveal that there is a need to ensure that the needs of transgender people are represented, fully integrated and clearly linked to outcomes that improve their health and quality of life.
    CONCLUSIONS: Discrimination experiences can result in poorer health outcomes; however, many people have developed resilience and positive coping strategies.
    CONCLUSIONS: Nurses need to recognise and respond appropriately to the care and treatment needs of this population. Comprehensive nursing assessments and plans of care that encompass all aspects of the person should be in place supported by clear policy guidelines and evidence-based research. The education requirements of practitioners are outlined.
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